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Product Info Summary
| SKU: | PA2019 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CETP Antibody Picoband®
SKU/Catalog Number
PA2019
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CETP Antibody catalog # PA2019. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CETP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2019)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CETP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2019 is reactive to CETP in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
54756 MW
Background of CETP
CETP (Cholesteryl Ester Transfer Protein Plasma), is a plasma protein that facilitates the transport of cholesteryl esters and triglycerides between the lipoproteins. CETP is also known as lipid transfer protein I (Day et al., 1994). Sparkes et al. (1987) used a CETP probe against DNA from a human/mouse somatic cell hybrid panel to assign the CETP gene to chromosome 16. Because the role of CETP in atherosclerosis remained unclear, Okamoto et al. (2000) attempted to develop a potent, specific CETP inhibitor. One inhibitor, JTT-705, forms a disulfide bond with CETP and increases high density lipoprotein (HDL) cholesterol, decreases non-HDL cholesterol, and inhibits the progression of atherosclerosis in rabbits.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2019 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Cell, COLO320 Cell, HT1080 Cell, JURKAT Cell, RAJI Cell, MCF-7 Cell
Validation Images & Assay Conditions
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Anti-CETP antibody, PA2019, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: RAJI Cell Lysate
Lane 6: MCF-7 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For CETP (Source: Uniprot.org, NCBI)
Gene Name
CETP
Full Name
Cholesteryl ester transfer protein
Weight
54756 MW
Superfamily
BPI/LBP/Plunc superfamily
Alternative Names
Cholesteryl ester transfer protein ;Lipid transfer protein I ;CETP ; CETP BPIFF, HDLCQ10 cholesteryl ester transfer protein cholesteryl ester transfer protein|BPI fold containing family F|cholesteryl ester transfer protein plasma|lipid transfer protein I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CETP, check out the CETP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CETP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CETP Antibody Picoband® (PA2019)
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No publications found for PA2019
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Anti-CETP Antibody Picoband®
Question
Does PA2019 anti-CETP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
As indicated on the product datasheet, PA2019 anti-CETP antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
The isotype is IgG, are you able to confirm that there are no IgA and IgM residues?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We are unable to confirm that there are no IgA and IgM residues.
Boster Scientific Support
Answered: 2020-03-03
Question
We need to test anti-CETP antibody PA2019 on human pancreas spleen for research purposes, then I may be interested in using anti-CETP antibody PA2019 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The products we sell, including anti-CETP antibody PA2019, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-21
Question
Is a blocking peptide available for product anti-CETP antibody (PA2019)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do provide the blocking peptide for product anti-CETP antibody (PA2019). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-30
Question
My question regarding product PA2019, anti-CETP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2019 anti-CETP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-17
Question
Will anti-CETP antibody PA2019 work for WB with pancreas spleen?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
According to the expression profile of pancreas spleen, CETP is highly expressed in pancreas spleen. So, it is likely that anti-CETP antibody PA2019 will work for WB with pancreas spleen.
Boster Scientific Support
Answered: 2018-06-25
Question
What is the purity of PA2019?
Verified Customer
Verified customer
Asked: 2015-06-17
Answer
The purity of PA2019 is >
Boster Scientific Support
Answered: 2015-06-17
Question
We are currently using anti-CETP antibody PA2019 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
M. Anderson
Verified customer
Asked: 2013-08-19
Answer
The anti-CETP antibody (PA2019) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-19
Question
See below the WB image, lot number and protocol we used for pancreas spleen using anti-CETP antibody PA2019. Please let me know if you require anything else.
D. Krishna
Verified customer
Asked: 2013-08-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-08
