Anti-Centromere protein W CENPW Antibody

Product Name

Anti-Centromere protein W CENPW Antibody

View all Cenpw Antibodies

SKU/Catalog Number

A07351

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Centromere protein W CENPW Antibody (Catalog # A07351). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CENPW antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Centromere protein W CENPW Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07351)

Host

Rabbit

Contents

CENPW Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW. The immunogen is located within the first 50 amino acids of CENPW.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of CENPW are known to exist; this antibody will detect all three isoforms.

Reactive Species

A07351 is reactive to CENPW in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

10061 MW

Background of Cenpw

CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07351 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CENPW (Source: Uniprot.org, NCBI)

Alternative Names

CUG2, CENP-W, C6orf173, CUG2, Centromere protein W, Cancer-up-regulated gene 2 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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