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Product Info Summary
| SKU: | A01035-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CDT1/DUP Antibody Picoband®
SKU/Catalog Number
A01035-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDT1/DUP Antibody Picoband® catalog # A01035-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CDT1/DUP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01035-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CDT1/DUP recombinant protein (Position: D508-L546).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01035-1 is reactive to CDT1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
60,390 MW
Background of Cdt1
CDT1 (Chromatin licensing and DNA replication factor 1) is a protein that in humans is encoded by the CDT1 gene. The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01035-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell lysates, human Hela whole cell lysates, human 293T whole cell lysates, rat liver tissue lysates, rat brain tissue lysates, mouse liver tissue lysates, mouse brain tissue lysates
ICC/IF: U2OS cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDT1/DUP using anti-CDT1/DUP antibody (A01035-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1/DUP antigen affinity purified polyclonal antibody (Catalog # A01035-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDT1/DUP at approximately 60 kDa. The expected band size for CDT1/DUP is at 60 kDa.
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Figure 2. IF analysis of CDT1/DUP using anti-CDT1/DUP antibody (A01035-1) and anti-Beta Tubulin antibody (M01857-3).
CDT1/DUP was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CDT1/DUP Antibody (A01035-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Donkey Anti-Rabbit IgG (BA1146) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of 293T cells using anti-CDT1/DUP antibody (A01035-1).
Overlay histogram showing 293T cells stained with A01035-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDT1/DUP Antibody (A01035-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CDT1 (Source: Uniprot.org, NCBI)
Gene Name
CDT1
Full Name
DNA replication factor Cdt1
Weight
60,390 MW
Superfamily
Cdt1 family
Alternative Names
Lumican; Keratan sulfate proteoglycan lumican; KSPG lumican; LUM; LDC; SLRR2D; Cdt1|2610318F11Rik, AW545653, C76791, DUP, R, Ris2|chromatin licensing and DNA replication factor 1|DNA replication factor Cdt1|double parked homolog|retroviral insertion site 2 protein|retroviral integration site 1|retroviral integration site 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDT1, check out the CDT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDT1/DUP Antibody Picoband® (A01035-1)
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