Anti-CDC5L Antibody

Product Name

Anti-CDC5L Antibody

View all CDC5L Antibodies

SKU/Catalog Number

PA2123

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDC5L Antibody catalog # PA2123. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC5L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2123)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CDC5L, different from the related rat and mouse sequences by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2123 is reactive to CDC5L in Human, Rat

Applications

PA2123 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

92251 MW

Background of CDC5L

Cell division cycle 5-like protein, also called PCDC5RP or CDC5-like, is a protein that in humans is encoded by the CDC5L gene. The CDC5L gene was mapped to chromosome 6p21 by fluorescence in situ hybridization. This DNA-binding protein involved in cell cycle control. It may act as a transcription activator. The Component of the PRP19-CDC5L complex forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The role of CDC5 (Polo-like kinase) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CDC5L using anti-CDC5L antibody (PA2123).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates
Lane 2: human THP-1 whole cell lysates
Lane 3: rat C6 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC5L antigen affinity purified polyclonal antibody (Catalog # PA2123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC5L at approximately 100KD. The expected band size for CDC5L is at 92KD.

Gene/Protein Information For CDC5L (Source: Uniprot.org, NCBI)

Alternative Names

CDC5 (cell division cycle 5, S. pombe, homolog)-like; CDC5 cell division cycle 5-like (S. pombe); CDC5; Cdc5-like protein; Cdc5-related protein; CEF1; cell division cycle 5-like protein; dJ319D22.1 (CDC5-like protein); dJ319D22.1; hCDC5; KIAA0432; PCDC5RPCDC5-LIKE; Pombe cdc5-related protein CDC5L CDC5, CDC5-LIKE, CEF1, PCDC5RP, dJ319D22.1 cell division cycle 5 like cell division cycle 5-like protein|CDC5 cell division cycle 5-like|Cdc5-related protein|dJ319D22.1 (CDC5-like protein)|pombe cdc5-related protein

For more info on CDC5L, check out the CDC5L Infographic

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In this infographic, you will see the following information for CDC5L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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