Product Info Summary
SKU: | M07786 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDC40 Rabbit Monoclonal Antibody
SKU/Catalog Number
M07786
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDC40 Rabbit Monoclonal Antibody catalog # M07786. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC40 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07786)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IAO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDC40
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07786 is reactive to CDC40 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
33 kDa
Calculated molecular weight
65521 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07786 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HeLa cell, K562 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CDC40 expression in (1) HeLa cell lysate; (2) K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDC40 (Source: Uniprot.org, NCBI)
Gene Name
CDC40
Full Name
Pre-mRNA-processing factor 17
Weight
65521 MW
Alternative Names
Pre-mRNA-processing factor 17;Cell division cycle 40 homolog;EH-binding protein 3;Ehb3;PRP17 homolog;hPRP17;CDC40;EHB3, PRP17, PRPF17; CDC40 EHB3, PRP17, PRPF17 cell division cycle 40 pre-mRNA-processing factor 17|EH-binding protein 3|PRP17 homolog|cell division cycle 40 homolog|hPRP17|pre-mRNA splicing factor 17
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC40, check out the CDC40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDC40 Rabbit Monoclonal Antibody (M07786)
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No publications found for M07786
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Customer Q&As
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6 Customer Q&As for Anti-CDC40 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-CDC40 Rabbit Monoclonal antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
As indicated on the product datasheet, M07786 anti-CDC40 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CDC40 Rabbit Monoclonal antibody M07786. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-04
Question
I am interested in to test anti-CDC40 Rabbit Monoclonal antibody M07786 on mouse liver for research purposes, then I may be interested in using anti-CDC40 Rabbit Monoclonal antibody M07786 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Wu
Verified customer
Asked: 2018-03-20
Answer
The products we sell, including anti-CDC40 Rabbit Monoclonal antibody M07786, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-20
Question
I have a question about product M07786, anti-CDC40 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M07786 anti-CDC40 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-12
Question
We are currently using anti-CDC40 Rabbit Monoclonal antibody M07786 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-17
Answer
The anti-CDC40 Rabbit Monoclonal antibody (M07786) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-17
Question
See attached the WB image, lot number and protocol we used for liver using anti-CDC40 Rabbit Monoclonal antibody M07786. Please let me know if you require anything else.
J. Collins
Verified customer
Asked: 2016-05-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-10