Product Info Summary
SKU: | A01343-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-cdc25C (Ab-216) Antibody
SKU/Catalog Number
A01343-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-cdc25C (Ab-216) Antibody (Catalog # A01343-1). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-cdc25C (Ab-216) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01343-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.214~218 (S-P-S-M-P) derived from Human cdc25C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01343-1 is reactive to CDC25C in Human
Applications
A01343-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
53365 MW
Background of CDC25C
cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded
protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348.
Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860.
Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677.
Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
![A01343 1 CDC25C WB test 1 A01343 1 CDC25C WB test 1](https://www.bosterbio.com/media/catalog/product/A/0/A01343-1-CDC25C-WB-test-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of CDC25C using anti-CDC25C antibody (A01343-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25C antigen affinity purified polyclonal antibody (Catalog # A01343-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDC25C.
![A01343 1 CDC25C IF test 3 A01343 1 CDC25C IF test 3](https://www.bosterbio.com/media/catalog/product/A/0/A01343-1-CDC25C-IF-test-3.jpg)
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using cdc25C(Ab-216) Antibody #A01343-1.
![A01343 1 CDC25C IHC test 2 A01343 1 CDC25C IHC test 2](https://www.bosterbio.com/media/catalog/product/A/0/A01343-1-CDC25C-IHC-test-2.jpg)
Click image to see more details
Figure 2. IHC analysis of CDC25C using anti-CDC25C antibody (A01343-1).
CDC25C was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDC25C Antibody (A01343-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CDC25C (Source: Uniprot.org, NCBI)
Gene Name
CDC25C
Full Name
M-phase inducer phosphatase 3
Weight
53365 MW
Superfamily
MPI phosphatase family
Alternative Names
CDC 25; CDC25; CDC25C; cell division cycle 25 homolog C (S. cerevisiae); cell division cycle 25 homolog C (S. pombe); cell division cycle 25C; Dual specificity phosphatase Cdc25C; EC 3.1.3.48; mitosis inducer CDC25; M-phase inducer phosphatase 3; phosphotyrosine phosphatase CDC25C CDC25, PPP1R60 cell division cycle 25C M-phase inducer phosphatase 3|CDC25 homolog C|dual specificity phosphatase CDC25C|mitosis inducer CDC25|phosphotyrosine phosphatase|protein phosphatase 1, regulatory subunit 60
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC25C, check out the CDC25C Infographic
![CDC25C infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC25C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-cdc25C (Ab-216) Antibody (A01343-1)
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