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Product Info Summary
| SKU: | P00209 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Xenopus |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-cdc2 (Tyr15) CDK1 Antibody
SKU/Catalog Number
P00209
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-cdc2 (Tyr15) CDK1 Antibody (Catalog # P00209). Tested in WB applications. This antibody reacts with Human, Mouse, Xenopus.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-cdc2 (Tyr15) CDK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00209)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat cdc2, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00209 is reactive to CDK1 in Human, Mouse, Xenopus
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
34095 MW
Background of CDC2/CDK1
Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr-15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003; Dranovsky et al., 2001; Okano et al., 1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00209 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in lanes 3 and 4. These blots are identical to the control and EGF treated except that they were incubated in gamma-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with gamma-Ptase.Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For CDK1 (Source: Uniprot.org, NCBI)
Gene Name
CDK1
Full Name
Cyclin-dependent kinase 1
Weight
34095 MW
Superfamily
protein kinase superfamily
Alternative Names
Cdc 2 antibody, Cdc2 antibody, CDC28A antibody, CDK-1 antibody, CDK 1 antibody, CDK1 antibody, CDK1_HUMAN antibody, CDKN1 antibody, CELL CYCLE CONTROLLER CDC2 antibody, Cell division control protein 2 antibody, Cell division control protein 2 homolog antibody, Cell division cycle 2 G1 to S and G2 to M antibody, Cell division protein kinase 1 antibody, Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, Cyclin-dependent kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, P34CDC2 antibody CDK1 CDC2, CDC28A, P34CDC2 cyclin dependent kinase 1 cyclin-dependent kinase 1|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK1, check out the CDK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-cdc2 (Tyr15) CDK1 Antibody (P00209)
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-cdc2 (Tyr15) CDK1 Antibody
Question
Do you have a BSA free version of anti-Phospho-cdc2 (Tyr15) antibody P00209 available?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-cdc2 (Tyr15) antibody P00209 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-16
Question
Can you help my question with product P00209, anti-Phospho-cdc2 (Tyr15) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00209 anti-Phospho-cdc2 (Tyr15) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-20
Question
Will P00209 anti-Phospho-cdc2 (Tyr15) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can see on the product datasheet, P00209 anti-Phospho-cdc2 (Tyr15) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-30
Question
We want to test anti-Phospho-cdc2 (Tyr15) antibody P00209 on xenopus intestine for research purposes, then I may be interested in using anti-Phospho-cdc2 (Tyr15) antibody P00209 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The products we sell, including anti-Phospho-cdc2 (Tyr15) antibody P00209, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-22
Question
Is a blocking peptide available for product anti-Phospho-cdc2 (Tyr15) antibody (P00209)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-Phospho-cdc2 (Tyr15) antibody (P00209). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for intestine using anti-Phospho-cdc2 (Tyr15) antibody P00209. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-10
Question
I see that the anti-Phospho-cdc2 (Tyr15) antibody P00209 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-25
Question
Is this P00209 anti-Phospho-cdc2 (Tyr15) antibody reactive to the isotypes of CDK1?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The immunogen of P00209 anti-Phospho-cdc2 (Tyr15) antibody is The antigen is a Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr15 of rat cdc2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-19
Question
We are currently using anti-Phospho-cdc2 (Tyr15) antibody P00209 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, xenopus. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The anti-Phospho-cdc2 (Tyr15) antibody (P00209) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
Does anti-Phospho-cdc2 (Tyr15) antibody P00209 work for WB with intestine?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
According to the expression profile of intestine, CDK1 is highly expressed in intestine. So, it is likely that anti-Phospho-cdc2 (Tyr15) antibody P00209 will work for WB with intestine.
Boster Scientific Support
Answered: 2019-03-22
Question
See attached the WB image, lot number and protocol we used for intestine using anti-Phospho-cdc2 (Tyr15) antibody P00209. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-07
Question
Our team were content with the WB result of your anti-Phospho-cdc2 (Tyr15) antibody. However we have seen positive staining in lymphoblast nucleus. cytoplasm. mitochondrion. using this antibody. Is that expected? Could you tell me where is CDK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
Based on literature, lymphoblast does express CDK1. Generally CDK1 expresses in nucleus. cytoplasm. mitochondrion. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-11
Question
We have observed staining in human intestine. Do you have any suggestions? Is anti-Phospho-cdc2 (Tyr15) antibody supposed to stain intestine positively?
C. Krishna
Verified customer
Asked: 2016-07-08
Answer
From literature intestine does express CDK1. From Uniprot.org, CDK1 is expressed in intestine, mammary cancer, skin, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CDK1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoblast, Pubmed ID: 14654843
Mammary cancer, Pubmed ID: 9515786, 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-07-08
Question
I was wanting to use your anti-Phospho-cdc2 (Tyr15) antibody for WB for xenopus intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for xenopus intestine identification?
C. Lewis
Verified customer
Asked: 2015-03-30
Answer
It shows on the product datasheet, P00209 anti-Phospho-cdc2 (Tyr15) antibody has been tested for WB on human, xenopus tissues. We have an innovator award program that if you test this antibody and show it works in xenopus intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-30
Question
My lab would like using your anti-Phospho-cdc2 (Tyr15) antibody for e2f-enabled inhibition of pre-replication complex formation studies. Has this antibody been tested with western blotting on human t47d cells? We would like to see some validation images before ordering.
D. Jones
Verified customer
Asked: 2013-06-13
Answer
Thank you for your inquiry. This P00209 anti-Phospho-cdc2 (Tyr15) antibody is validated on human t47d cells. It is guaranteed to work for WB in human, xenopus. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-06-13
