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Product Info Summary
| SKU: | A00196 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CD80 Antibody
SKU/Catalog Number
A00196
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CD80 Antibody (Catalog # A00196). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CD80 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00196)
Host
Rabbit
Contents
CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CD80 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human CD80. The immunogen is located within amino acids 60 - 110 of CD80.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
Reactive Species
A00196 is reactive to CD80 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
33048 MW
Background of B7-1/CD80
CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00196 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CD80 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CD80 in EL4 cell lysate with CD80 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 20 μg/ml.
Red: CD80 Antibody (A00196)
Blue: DAPI staining
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Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 2 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)
Gene Name
CD80
Full Name
T-lymphocyte activation antigen CD80
Weight
33048 MW
Alternative Names
CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation CD80|B-lymphocyte activation B7|CD80 (CD28 ligand 1, B7-1 )|CTLA-4 counter-receptor B7.1|activation B7-1 |costimulatory factor CD80|costimulatory molecule variant IgV-CD80
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD80, check out the CD80 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD80 Antibody (A00196)
Hello CJ!
A00196 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L, Zheng Y,Sun Y.ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.Theranostics.
Species: Human,Mouse
A00196 usage in article: APP:IF, SAMPLE:TUMOR CELL LINES, DILUTION:1:100
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