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Product Info Summary
| SKU: | A01974-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cd7 Antibody Picoband®
SKU/Catalog Number
A01974-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cd7 Antibody Picoband® catalog # A01974-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cd7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01974-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cd7 recombinant protein (Position: Q24-Q210).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01974-3 is reactive to Cd7 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
30570 MW
Background of CD7
CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01974-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat thymus tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cd7 using anti-Cd7 antibody (A01974-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd7 antigen affinity purified polyclonal antibody (Catalog # A01974-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd7 at approximately 36 kDa. The expected band size for Cd7 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Cd7 (Source: Uniprot.org, NCBI)
Gene Name
Cd7
Full Name
T-cell antigen CD7
Weight
30570 MW
Alternative Names
T-cell antigen CD7; GP40; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41; CD7; CD7 CD7 GP40, LEU-9, TP41, Tp40 CD7 molecule T-cell CD7|CD7 (p41)|T-cell leukemia |T-cell surface Leu-9|p41 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd7, check out the Cd7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cd7 Antibody Picoband® (A01974-3)
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