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Product Info Summary
| SKU: | M01974 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD7 Monoclonal Antibody
SKU/Catalog Number
M01974
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD7 Monoclonal Antibody catalog # M01974. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01974)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADIG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD7 Not yet known.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01974 is reactive to CD7 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
18473 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01974 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human PBMC
Validation Images & Assay Conditions
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Western blot analysis of CD7 expression in human PBMC lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD7 (Source: Uniprot.org, NCBI)
Gene Name
CD7
Full Name
T-cell antigen CD7
Weight
18473 MW
Alternative Names
Ubiquitin D;Diubiquitin;Ubiquitin-like protein FAT10;UBD;FAT10; CD7 GP40, LEU-9, TP41, Tp40 CD7 molecule T-cell CD7|CD7 (p41)|T-cell leukemia |T-cell surface Leu-9|p41 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD7, check out the CD7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD7 Monoclonal Antibody (M01974)
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No publications found for M01974
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD7 Monoclonal Antibody
Question
Is there a BSA free version of anti-CD7 Monoclonal antibody M01974 available?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD7 Monoclonal antibody M01974 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-05
Question
Does M01974 anti-CD7 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
You can see on the product datasheet, M01974 anti-CD7 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-02
Question
I was wanting to use your anti-CD7 Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
It shows on the product datasheet, M01974 anti-CD7 Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-06
Question
Is this M01974 anti-CD7 Monoclonal antibody reactive to the isotypes of CD7?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The immunogen of M01974 anti-CD7 Monoclonal antibody is A synthesized peptide derived from human CD7 Not yet known. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-31
Question
I am looking for to test anti-CD7 Monoclonal antibody M01974 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD7 Monoclonal antibody M01974 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The products we sell, including anti-CD7 Monoclonal antibody M01974, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-10
Question
We are currently using anti-CD7 Monoclonal antibody M01974 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-CD7 Monoclonal antibody (M01974) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
Question
We ordered your anti-CD7 Monoclonal antibody for IP on liver a few years ago. I am using human, and We intend to use the antibody for WB next. I am interested in examining liver as well as muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-18
Answer
I looked at the website and datasheets of our anti-CD7 Monoclonal antibody and it appears that M01974 has been tested on human in both IP and WB. Thus M01974 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-18
Question
Would anti-CD7 Monoclonal antibody M01974 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
According to the expression profile of leukemic t-cell, CD7 is highly expressed in leukemic t-cell. So, it is likely that anti-CD7 Monoclonal antibody M01974 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2018-03-22
Question
We have observed staining in human liver. Any tips? Is anti-CD7 Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
From what I have seen in literature liver does express CD7. From what I have seen in Uniprot.org, CD7 is expressed in blood, muscle, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD7 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-11
Question
Our lab were happy with the WB result of your anti-CD7 Monoclonal antibody. However we have seen positive staining in leukemic t-cell membrane using this antibody. Is that expected? Could you tell me where is CD7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
From what I have seen in literature, leukemic t-cell does express CD7. Generally CD7 expresses in membrane. Regarding which tissues have CD7 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD7 Monoclonal antibody M01974. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-06
Question
Is a blocking peptide available for product anti-CD7 Monoclonal antibody (M01974)?
R. Gonzalez
Verified customer
Asked: 2016-02-01
Answer
We do provide the blocking peptide for product anti-CD7 Monoclonal antibody (M01974). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-01
Question
I have a question about product M01974, anti-CD7 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Walker
Verified customer
Asked: 2015-08-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01974 anti-CD7 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-31
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-CD7 Monoclonal antibody M01974. Please let me know if you require anything else.
M. Evans
Verified customer
Asked: 2014-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-03
Question
I see that the anti-CD7 Monoclonal antibody M01974 works with IF, what is the protocol used to produce the result images on the product page?
C. Dhar
Verified customer
Asked: 2013-04-22
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-22
