Anti-CD64/FCGR1A Antibody Picoband™

Product Name

Anti-CD64/FCGR1A Antibody Picoband™

View all Fc gamma RI/CD64 Antibodies

SKU/Catalog Number

A02428-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD64/FCGR1A Antibody Picoband™ catalog # A02428-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CD64/FCGR1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02428-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CD64/FCGR1A recombinant protein (Position: A191-D254).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02428-3 is reactive to FCGR1A in Human, Mouse, Rat

Applications

A02428-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

38779 MW

Background of Fc gamma RI/CD64

High affinity immunoglobulin gamma Fc receptor I is a protein that in humans is encoded by the FCGR1A gene. It is mapped to 1q21.2. This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CD64/FCGR1A using anti-CD64/FCGR1A antibody (A02428-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse EL-4 whole cell lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates,
Lane 9: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD64/FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD64/FCGR1A at approximately 70 kDa. The expected band size for CD64/FCGR1A is at 43 kDa.

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Figure 2. Flow Cytometry analysis of SiHa cells using anti-CD64/FCGR1A antibody (A02428-3).
Overlay histogram showing SiHa cells stained with A02428-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD64/FCGR1A Antibody (A02428-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For FCGR1A (Source: Uniprot.org, NCBI)

Alternative Names

CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I FCGR1A CD64, CD64A, FCRI, IGFR1 Fc fragment of IgG receptor Ia high affinity immunoglobulin gamma Fc receptor I|Fc fragment of IgG, high affinity Ia, receptor (CD64)|Fc fragment of IgG, high affinity Ia, receptor for (CD64)|Fc gamma receptor Ia|Fc-gamma RI|Fc-gamma receptor I A1|IgG Fc receptor I|fc-gamma RIA|fcgammaRIa

For more info on FCGR1A, check out the FCGR1A Infographic

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In this infographic, you will see the following information for FCGR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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