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Product Info Summary
| SKU: | M00171 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, Functional Assay |
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Product info
Product Name
Anti-CD54 / ICAM-1 Monoclonal Antibody
View all ICAM-1/CD54 Antibodies
SKU/Catalog Number
M00171
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD54 / ICAM-1 Monoclonal Antibody (Catalog # M00171). Tested in Functional Assay, Flow Cytometry, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD54 / ICAM-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00171)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: W-CAM-1; same as Wehi-CAM-1 or 1H4
Isotype
IgG2b, kappa
Immunogen
Raji Burkitt lymphoma cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00171 is reactive to ICAM1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
57825 MW
Background of ICAM-1/CD54
Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of Purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This monoclonal antibody blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00171 is guaranteed for Flow Cytometry, IF, IHC, Functional Assay Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-CD54 Monoclonal Antibody (W-CAM-1).
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SDS-PAGE analysis purified Anti-CD54 Monoclonal Antibody (W-CAM-1). Confirmation of integrity and purity of antibody.
Protein Target Info & Infographic
Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)
Gene Name
ICAM1
Full Name
Intercellular adhesion molecule 1
Weight
57825 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Intercellular adhesion molecule 1;ICAM-1;Major group rhinovirus receptor;CD54;ICAM1; ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICAM1, check out the ICAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD54 / ICAM-1 Monoclonal Antibody (M00171)
Hello CJ!
M00171 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells
Cheng Y, Zhang Y, Song H, Feng J. Neural Regen Res. 2012 Jun 15;7(17):1312-7. Doi: 10.3969/J.Issn.1673-5374.2012.17.005. Intercellular Adhesion Molecule-1 Expression In The Hippocampal Ca1 Region Of Hyperlipidemic Rats With Chronic Cerebral Ischemia.
Yao Jh, Zhang Xs, Zheng Ss, Li Yh, Wang Lm, Wang Zz, Chu L, Hu Xw, Liu Kx, Tian Xf. World J Gastroenterol. 2009 Jul 14;15(26):3240-5. Prophylaxis With Carnosol Attenuates Liver Injury Induced By Intestinal Ischemia/Reperfusion.
Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H. Crit Care. 2013 Mar 11;17(2):R44. Doi: 10.1186/Cc12558. Glycyrrhizin Protects Against Porcine Endotoxemia Through Modulation Of Systemic Inflammatory Response.
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Customer Q&As
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3 Customer Q&As for Anti-CD54 / ICAM-1 Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-CD54 / ICAM-1 Monoclonal antibody. However we have been able to see positive staining in blood membrane using this antibody. Is that expected? Could you tell me where is ICAM1 supposed to be expressed?
C. Wu
Verified customer
Asked: 2019-06-20
Answer
Based on literature, blood does express ICAM1. Generally ICAM1 expresses in membrane. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-06-20
Question
We have seen staining in human cervix carcinoma. What should we do? Is anti-CD54 / ICAM-1 Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
According to literature cervix carcinoma does express ICAM1. According to Uniprot.org, ICAM1 is expressed in lung, kidney, blood, plasma, cervix carcinoma, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-05-11
Question
We are currently using anti-CD54 / ICAM-1 Monoclonal antibody M00171 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Z. Patel
Verified customer
Asked: 2014-04-28
Answer
The anti-CD54 / ICAM-1 Monoclonal antibody (M00171) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-28
