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Product Info Summary
| SKU: | M06249 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD53 Monoclonal Antibody
SKU/Catalog Number
M06249
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD53 Monoclonal Antibody catalog # M06249. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD53 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06249)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFFG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD53 May be involved in growth regulation in hematopoietic cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06249 is reactive to CD53 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
60 kDa
Calculated molecular weight
39183 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06249 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of CD53 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD53 (Source: Uniprot.org, NCBI)
Gene Name
CD53
Full Name
Leukocyte surface antigen CD53
Weight
39183 MW
Superfamily
tetraspanin (TM4SF) family
Alternative Names
Histone deacetylase 11;HD11;3.5.1.98;HDAC11; CD53 MOX44, TSPAN25 CD53 molecule leukocyte surface CD53|CD53 |CD53 glycoprotein|CD53 tetraspan | MOX44 by MRC-OX44|cell surface |cell surface glycoprotein CD53|tetraspanin-25|transmembrane glycoprotein|tspan-25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD53, check out the CD53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD53 Monoclonal Antibody (M06249)
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No publications found for M06249
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CD53 Monoclonal Antibody
Question
Why the expected band size of 24 kDa can't be seen. Instead there is a band showing up at approx. 35 kDa. What is the cause?
Verified customer
Asked: 2022-01-26
Answer
M06249 is a membrane protein, and it will have post-translational modifications that cause the actual molecular weight to be larger than theoretical MW.
Boster Scientific Support
Answered: 2022-01-27
Question
I see that the anti-CD53 Monoclonal antibody M06249 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-04
Question
Is a blocking peptide available for product anti-CD53 Monoclonal antibody (M06249)?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
We do provide the blocking peptide for product anti-CD53 Monoclonal antibody (M06249). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-07
Question
We are currently using anti-CD53 Monoclonal antibody M06249 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
C. Gonzalez
Verified customer
Asked: 2016-06-30
Answer
The anti-CD53 Monoclonal antibody (M06249) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-30
