Anti-CD45/PTPRC Antibody

PTPRC antibody

Boster Bio Anti-CD45/PTPRC Antibody catalog # PA1596. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 6 publication(s).

Product Info Summary

SKU: PA1596
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-CD45/PTPRC Antibody

View all PTPRC Antibodies

SKU/Catalog Number

PA1596

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD45/PTPRC Antibody catalog # PA1596. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD45/PTPRC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1596)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD45.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1596 is reactive to PTPRC in Human

Applications

PA1596 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

147 kDa

Calculated molecular weight

147.486kDa

Background of PTPRC

CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunofluorescence, 2μg/ml, Human, -

Validation Images & Assay Conditions

Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)

Gene Name

PTPRC

Full Name

Receptor-type tyrosine-protein phosphatase C

Weight

147.486kDa

Superfamily

protein-tyrosine phosphatase family

Alternative Names

B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|protein tyrosine phosphatase, receptor type, c polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTPRC, check out the PTPRC Infographic

PTPRC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1596 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment

Fas counterattack in cholangiocarcinoma: A mechanism for immune evasion in human hilar cholangiocarcinomas

Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells

Huang Y, Jia X, Bai K, Gong X, Fan Y. Arch Med Res. 2010 Oct;41(7):497-505. Doi: 10.1016/J.Arcmed.2010.10.002. Effect Of Fluid Shear Stress On Cardiomyogenic Differentiation Of Rat Bone Marrow Mesenchymal Stem Cells.

Liu D, Wang Y, Ye Y, Yin G, Chen L. Cell Immunol. 2014 May-Jun;289(1-2):7-14. Doi: 10.1016/J.Cellimm.2014.01.007. Epub 2014 Feb 2. Distinct Molecular Basis For Endothelial Differentiation: Gene Expression Profiles Of Human Mesenchymal Stem Cells V...

Li X, Huang Y, Zheng L, Liu H, Niu X, Huang J, Zhao F, Fan Y. J Biomed Mater Res A. 2014 Apr;102(4):1092-101. Doi: 10.1002/Jbm.A.34774. Epub 2013 Jun 11. Effect Of Substrate Stiffness On The Functions Of Rat Bone Marrow And Adipose Tissue Derived ...

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5 Customer Q&As for Anti-CD45/PTPRC Antibody

Question

Our lab were well pleased with the WB result of your anti-CD45/PTPRC antibody. However we have seen positive staining in t-cell cell membrane using this antibody. Is that expected? Could you tell me where is PTPRC supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

From literature, t-cell does express PTPRC. Generally PTPRC expresses in cell membrane. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2020-03-31

Question

you antibody using your anti-CD45/PTPRC antibody for positive regulation of b cell proliferation studies. Has this antibody been tested with western blotting on cem whole cell lysate? We would like to see some validation images before ordering.

K. Jha

Verified customer

Asked: 2019-11-12

Answer

We appreciate your inquiry. This PA1596 anti-CD45/PTPRC antibody is validated on jurkat whole cell lysate, cem whole cell lysate, human tonsil tissue. It is guaranteed to work for IF, IHC-P, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-12

Question

We have seen staining in human t-cell. Are there any suggestions? Is anti-CD45/PTPRC antibody supposed to stain t-cell positively?

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

From literature t-cell does express PTPRC. From Uniprot.org, PTPRC is expressed in blood, lymphocyte, synovium, placenta, t-cell, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2019-06-19

Question

We are currently using anti-CD45/PTPRC antibody PA1596 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The anti-CD45/PTPRC antibody (PA1596) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-09

Question

We ordered your anti-CD45/PTPRC antibody for ICC on placenta in a previous project. I am using human, and We intend to use the antibody for IF next. I am interested in examining placenta as well as lymphocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

P. Jones

Verified customer

Asked: 2018-11-26

Answer

I took a look at the website and datasheets of our anti-CD45/PTPRC antibody and I see that PA1596 has been tested on human in both ICC and IF. Thus PA1596 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-11-26

Order DetailsPrice
PA1596

100μg

$370
PA1596-10ug

10μg sample (liquid)

$99
PA1596-Biotin

100 μg Biotin conjugated

$570
PA1596-Cy3

100 μg Cy3 conjugated

$570
PA1596-Dylight488

100 μg Dylight488 conjugated

$570
PA1596-Dylight550

100 μg Dylight550 conjugated

$570
PA1596-Dylight594

100 μg Dylight594 conjugated

$570
PA1596-FITC

100 μg FITC conjugated

$570
PA1596-HRP

100 μg HRP conjugated

$570
PA1596-APC

100 μg APC conjugated

$670
PA1596-PE

100 μg PE conjugated

$670
PA1596-carrier-free

Carrier Free

$370

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