Click image to see more details
Product Info Summary
| SKU: | FC00555-FITC |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated
SKU/Catalog Number
FC00555-FITC
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC00555-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00555-FITC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: F10-89-4
Isotype
IgG2a,k
Immunogen
Human T lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00555-FITC is reactive to PTPRC in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
147254 MW
Background of PTPRC
CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00555-FITC is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
PBMCs lymphocytes stained with FITC conjugated anti-human CD45 (clone F10- 89-4) (red histogram). PBMCs lymphocytes stained with isotype control IgG1 MOPC- 31C FITC (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147254 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 |T200 glycoprotein|T200 leukocyte common |protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated (FC00555-FITC)
Hello CJ!
FC00555-FITC has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model
Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis
Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis
Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated
Question
Can FC00052-FITC, FC00555-FITC and FC01818-FITC be used on porcine?
Verified customer
Asked: 2019-03-28
Answer
Unfortunately, we do not have any data regarding potential porcine cross-reactivity for the Anti-Human CD44 Monoclonal Antibody FITC Conjugated, Flow Validated (FC00052-FITC), Anti-Human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated (FC00555-FITC) and Anti-Human CD90 THY1 Monoclonal Antibody FITC Conjugated, Flow Validated (FC01818-FITC).
Boster Scientific Support
Answered: 2019-04-01
