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Product Info Summary
| SKU: | M00344 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00344
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD4 Rabbit Monoclonal Antibody catalog # M00344. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00344)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00344 is reactive to CD4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated molecular weight
51297 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00344 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
IHC: human appendix tissue, human appendix tissue, human appendix tissue, human appendix tissue, human tonsil tissue, human tonsil tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of CD4 expression in THP-1 cell lysate.
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Figure 2. IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD4 (Source: Uniprot.org, NCBI)
Gene Name
CD4
Full Name
T-cell surface glycoprotein CD4
Weight
51297 MW
Alternative Names
T-cell surface glycoprotein CD4;T-cell differentiation antigen L3T4;T-cell surface antigen T4/Leu-3;CD4;Cd4; CD4 CD4mut CD4 molecule T-cell surface glycoprotein CD4|CD4 (p55)|CD4 receptor|T-cell surface T4/Leu-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD4, check out the CD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD4 Rabbit Monoclonal Antibody (M00344)
Hello CJ!
M00344 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ouyang W, An T, Guo D, Wu S, Tien P. Aids Res Hum Retroviruses. 2013 Mar;29(3):613-20. Doi: 10.1089/Aid.2012.0059. Epub 2013 Jan 11. The Potent Human Immunodeficiency Virus Type 1 (Hiv-1) Entry Inhibitor Hr212 Blocks Formation Of The Envelope Glyc...
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Customer Q&As
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4 Customer Q&As for Anti-CD4 Rabbit Monoclonal Antibody
Question
We are currently using anti-CD4 Rabbit Monoclonal antibody M00344 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The anti-CD4 Rabbit Monoclonal antibody (M00344) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-08
Question
We ordered your anti-CD4 Rabbit Monoclonal antibody for WB on thymus in a previous experiment. I am using human, and I plan to use the antibody for IF next. My question regards examining thymus as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
I looked at the website and datasheets of our anti-CD4 Rabbit Monoclonal antibody and it seems that M00344 has been tested on human in both WB and IF. Thus M00344 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-03
Question
Our team were happy with the WB result of your anti-CD4 Rabbit Monoclonal antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CD4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Based on literature, brain does express CD4. Generally CD4 expresses in cell membrane. Regarding which tissues have CD4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1425921
Brain, Pubmed ID: 9074930
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-22
Question
We have been able to see staining in human blood. Any tips? Is anti-CD4 Rabbit Monoclonal antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
From literature blood does express CD4. From Uniprot.org, CD4 is expressed in leukocyte, brain, thymus, pancreas, blood, liver, among other tissues. Regarding which tissues have CD4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1425921
Brain, Pubmed ID: 9074930
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-07-28
