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Product Info Summary
| SKU: | FC02675-PE |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD3 Cd3e Monoclonal Antibody PE Conjugated, Flow Validated
SKU/Catalog Number
FC02675-PE
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD3 Cd3e Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC02675-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD3 Cd3e Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02675-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: OKT3
Isotype
IgG2a,k
Immunogen
Human peripheral blood lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC02675-PE is reactive to Cd3e in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
21312 MW
Background of CD3E
The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC02675-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated PBMCs stained with anti-human CD3 PE (clone OKT3, red histogram). PBMCs lymphocytes stained mouse IgG2a PE isotype control (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For Cd3e (Source: Uniprot.org, NCBI)
Gene Name
Cd3e
Full Name
T-cell surface glycoprotein CD3 epsilon chain
Weight
21312 MW
Alternative Names
CD3 antigen, epsilon polypeptide (Predicted) ;CD3e molecule ;Cd3e ;Cd3e_predicted ;rCG_58471 ; CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e , epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell receptor complex, epsilon subunit of T3|T-cell surface T3/Leu-4 epsilon chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd3e, check out the Cd3e Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd3e: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD3 Cd3e Monoclonal Antibody PE Conjugated, Flow Validated (FC02675-PE)
Hello CJ!
FC02675-PE has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-human CD3 Cd3e Monoclonal Antibody PE Conjugated, Flow Validated
Question
I would like to test anti-human CD3 Monoclonal antibody PE Conjugated, Flow Validated FC02675-PE on human thymus for research purposes, then I may be interested in using anti-human CD3 Monoclonal antibody PE Conjugated, Flow Validated FC02675-PE for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The products we sell, including anti-human CD3 Monoclonal antibody PE Conjugated, Flow Validated FC02675-PE, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-22
Question
I was wanting to use your anti-human CD3 Monoclonal antibody PE Conjugated, Flow Validated for Flow Cytometry for human thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thymus identification?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can see on the product datasheet, FC02675-PE anti-human CD3 Monoclonal antibody PE Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-13
