Anti-CD370 Purified CLEC9A Monoclonal Antibody

Product Name

Anti-CD370 Purified CLEC9A Monoclonal Antibody

View all Clec9a Antibodies

SKU/Catalog Number

M04577

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CD370 Purified CLEC9A Monoclonal Antibody (Catalog# M04577). Tested in Flow Cytometry, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-CD370 Purified CLEC9A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04577)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

8F9

Isotype

Mouse IgG2a

Immunogen

RBL-2H3 cells expressing human CLEC9A fused to an HA epitope. The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04577 is reactive to CLEC9A in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

27324 MW

Background of Clec9a

CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04577 is guaranteed for Flow Cytometry, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For CLEC9A (Source: Uniprot.org, NCBI)

Alternative Names

CLEC9A, DNGR1, DNGR-1, UNQ9341 Clec9a|9830005G06Rik, DNGR, DNGR-1|C-type lectin domain family 9, member a|C-type lectin domain family 9 member A|dendritic cell natural killer lectin group receptor 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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