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Product Info Summary
| SKU: | A02995-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Scramblase 1/PLSCR1 Antibody Picoband®
View all Phospholipid Scramblase 1/PLSCR1 Antibodies
SKU/Catalog Number
A02995-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Scramblase 1/PLSCR1 Antibody Picoband® catalog # A02995-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Scramblase 1/PLSCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02995-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Scramblase 1/PLSCR1 recombinant protein (Position: M1-S54).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02995-2 is reactive to PLSCR1 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
36020 MW
Background of Phospholipid Scramblase 1/PLSCR1
Phospholipid scramblase 1 is a protein that in human is encoded by the PLSCR1 gene. This gene is mapped to 3q24.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02995-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human CACO-2 whole cell, human Hela whole cell, human A431 whole cell, human 293T whole cell, mouse lung tissue, mouse RAW2647 whole cell
IHC: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PLSCR1 using anti-PLSCR1 antibody (A02995-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLSCR1 antigen affinity purified polyclonal antibody (Catalog # A02995-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLSCR1 at approximately 35 kDa. The expected band size for PLSCR1 is at 35 kDa.
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Figure 2. IHC analysis of PLSCR1 using anti-PLSCR1 antibody (A02995-2).
PLSCR1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLSCR1 Antibody (A02995-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PLSCR1 (Source: Uniprot.org, NCBI)
Gene Name
PLSCR1
Full Name
Phospholipid scramblase 1
Weight
36020 MW
Superfamily
phospholipid scramblase family
Alternative Names
Phospholipid scramblase 1; PL scramblase 1; Ca (2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b; PLSCR1 PLSCR1 MMTRA1B phospholipid scramblase 1 phospholipid scramblase 1|PL scramblase 1|ca(2+)-dependent phospholipid scramblase 1|erythrocyte phospholipid scramblase|mg(2+)-dependent nuclease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLSCR1, check out the PLSCR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLSCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Scramblase 1/PLSCR1 Antibody Picoband® (A02995-2)
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