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Product Info Summary
| SKU: | M01513 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Cynomolgus monkey, Human, Rabbit |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody
View all CD31/PECAM-1 Antibodies
SKU/Catalog Number
M01513
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (Catalog # M01513). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Cynomolgus monkey, Rabbit.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM122
Isotype
IgG1, kappa
Immunogen
Membrane preparation of a spleen from a patient with hairy cell leukemia
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01513 is reactive to PECAM1 in Cynomolgus monkey, Human, Rabbit
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
82536 MW
Background of CD31/PECAM-1
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01513 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Anti-CD31 Monoclonal Antibody (SPM122)
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SDS-PAGE analysis of Anti-CD31 Antibody [SPM122] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Protein Target Info & Infographic
Gene/Protein Information For PECAM1 (Source: Uniprot.org, NCBI)
Gene Name
PECAM1
Full Name
Platelet endothelial cell adhesion molecule
Weight
82536 MW
Alternative Names
Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA';PECA1;CD31;PECAM1; PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 |platelet endothelial cell adhesion molecule-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PECAM1, check out the PECAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PECAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (M01513)
Hello CJ!
M01513 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miR‑126 regulates the progression of epithelial ovarian cancer in vitro and in vivo by targeting VEGF‑A
Construction of tissue‑engineered bone using a bioreactor and platelet‑rich plasma
Relationship Between Apoptosis of Endplate Microvasculature and Degeneration of the Intervertebral Disk
In situ production of pre-vascularized synthetic bone grafts for regenerating critical-sized defects in rabbits
Wang B,Lv X,Li Z,Zhang M,Yao J,Sheng N,Lu M,Wang H,Chen S.Urethra-inspired biomimetic scaffold: A therapeutic strategy to promote angiogenesis for urethral regeneration in a rabbit model.Acta Biomater.2020 Jan 15;102:247-258.doi:10.1016/j.actbio.2019.11.0
Species: Human,New Zealand Rabbit
M01513 usage in article: APP:IHC, SAMPLE:EPITHELIAL CELL LAYERS AND BLOOD VESSELS, DILUTION:NA
Liu H,Chen W,Zhao B,Quan W,Zhang Y,Zhou Y,Wan Z,Zhang X,Xue G,Li J,Luo S,Wang J,Liu Y,Zhen M,Zhao Y.Autologous bionic tissue for inguinal hernia repair.J Biomed Mater Res A.2020 Jun; 108(6):1351-1368.doi:10.1002/jbm.a.36907.Epub 2020 Mar 2.PMID:32090432.
Species: New Zealand white Rabbit
M01513 usage in article: APP:IHC, SAMPLE:MSCS, DILUTION:NA
EPCR promotes MGC803 human gastric cancer cell tumor angiogenesis in vitro through activating ERK1/2 and AKT in a PAR1%u2011dependent manner
The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex
Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling
ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-07
Question
Can you help my question with product M01513, anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01513 anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-04
Question
I see that the anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-27
Question
Please see the WB image, lot number and protocol we used for liver using anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-02
Question
Does M01513 anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
As indicated on the product datasheet, M01513 anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-29
Question
Is a blocking peptide available for product anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody (M01513)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We do provide the blocking peptide for product anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody (M01513). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-19
Question
I am looking for to test anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 on monkey liver for research purposes, then I may be interested in using anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The products we sell, including anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-30
Question
Our lab were content with the WB result of your anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody. However we have observed positive staining in platelet cell membrane using this antibody. Is that expected? Could you tell me where is PECAM1 supposed to be expressed?
B. Jones
Verified customer
Asked: 2019-08-08
Answer
Based on literature, platelet does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-08-08
Question
We are currently using anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 for rabbit tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, monkey, rabbit. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
The anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody (M01513) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-23
Question
We have been able to see staining in human brain. Do you have any suggestions? Is anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
From literature brain does express PECAM1. From Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2018-03-02
Question
Is this M01513 anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody reactive to the isotypes of PECAM1?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
The immunogen of M01513 anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody is Membrane preparation of a spleen from a patient with hairy cell leukemia. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-28
Question
Does anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 work for IHC with liver?
J. Taylor
Verified customer
Asked: 2017-01-05
Answer
According to the expression profile of liver, PECAM1 is highly expressed in liver. So, it is likely that anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 will work for IHC with liver.
Boster Scientific Support
Answered: 2017-01-05
Question
Do you have a BSA free version of anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 available?
B. Singh
Verified customer
Asked: 2016-07-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody M01513 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-07-22
Question
We ordered your anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody for IHC on liver in a previous experiment. I am using monkey, and We are going to use the antibody for WB next. My question regards examining liver as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for WB?
T. Baker
Verified customer
Asked: 2014-06-10
Answer
I looked at the website and datasheets of our anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody and it seems that M01513 has been tested on monkey in both IHC and WB. Thus M01513 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in monkey even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-06-10
Question
I was wanting to use your anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody for IHC for monkey liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for monkey liver identification?
E. Zhao
Verified customer
Asked: 2013-09-24
Answer
You can see on the product datasheet, M01513 anti-CD31 / PECAM-1 (Endothelial Cell Marker) Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, WB on human, monkey, rabbit tissues. We have an innovator award program that if you test this antibody and show it works in monkey liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-24
