Product Info Summary
SKU: | A10095 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-CD302/Dcl 1 Antibody
View all CD302/CLEC13A Antibodies
SKU/Catalog Number
A10095
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CD302/Dcl 1 Antibody (Catalog # A10095). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD302/Dcl 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10095)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CD302.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10095 is reactive to CD302 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
26183 MW
Background of CD302/CLEC13A
Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration.
Kato M., J. Biol. Chem. 278:34035-34041(2003).
Hillier L.W., Nature 434:724-731(2005).
Nomura N., DNA Res. 1:27-35(1994).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10095 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using CD302 antibody A10095.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using CD302 antibody A10095.
Protein Target Info & Infographic
Gene/Protein Information For CD302 (Source: Uniprot.org, NCBI)
Gene Name
CD302
Full Name
CD302 antigen
Weight
26183 MW
Alternative Names
CD302 antigen;C-type lectin BIMLEC;C-type lectin domain family 13 member A;DEC205-associated C-type lectin 1;Type I transmembrane C-type lectin receptor DCL-1;CD302;CD302;CLEC13A, DCL1, KIAA0022; CD302 BIMLEC, CLEC13A, DCL-1, DCL1 CD302 molecule CD302 |C-type lectin domain family 13, member A|DEC205-associated C-type lectin 1|type I transmembrane C-type lectin receptor DCL-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD302, check out the CD302 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD302: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD302/Dcl 1 Antibody (A10095)
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No publications found for A10095
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Customer Q&As
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7 Customer Q&As for Anti-CD302/Dcl 1 Antibody
Question
I was wanting to use your anti-CD302/Dcl 1 antibody for IHC for mouse bone marrow on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
It shows on the product datasheet, A10095 anti-CD302/Dcl 1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-22
Question
We are currently using anti-CD302/Dcl 1 antibody A10095 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
L. Zhang
Verified customer
Asked: 2018-12-11
Answer
The anti-CD302/Dcl 1 antibody (A10095) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-11
Question
Does anti-CD302/Dcl 1 antibody A10095 work on zebrafish IHC with bone marrow?
A. Evans
Verified customer
Asked: 2018-08-01
Answer
Our lab technicians have not validated anti-CD302/Dcl 1 antibody A10095 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD302/Dcl 1 antibody A10095 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish bone marrow in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-01
Question
See attached the WB image, lot number and protocol we used for bone marrow using anti-CD302/Dcl 1 antibody A10095. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-18
Question
We need to test anti-CD302/Dcl 1 antibody A10095 on mouse bone marrow for research purposes, then I may be interested in using anti-CD302/Dcl 1 antibody A10095 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Parker
Verified customer
Asked: 2018-05-21
Answer
The products we sell, including anti-CD302/Dcl 1 antibody A10095, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-21
Question
I see that the anti-CD302/Dcl 1 antibody A10095 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-09
Question
Is a blocking peptide available for product anti-CD302/Dcl 1 antibody (A10095)?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
We do provide the blocking peptide for product anti-CD302/Dcl 1 antibody (A10095). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-28