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Product Info Summary
| SKU: | A10132 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-CD300b CD300LB Antibody
View all CD300b/LMIR5/CD300LB Antibodies
SKU/Catalog Number
A10132
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD300b CD300LB Antibody catalog # A10132. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD300b CD300LB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10132)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 61-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10132 is reactive to CD300LB in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
22689 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10132 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For CD300LB (Source: Uniprot.org, NCBI)
Gene Name
CD300LB
Full Name
CMRF35-like molecule 7
Weight
22689 MW
Superfamily
CD300 family
Alternative Names
CMRF35-like molecule 7;CLM-7;CD300 antigen-like family member B;CMRF35-A2;Immune receptor expressed on myeloid cells 3;IREM-3;Leukocyte mono-Ig-like receptor 5;Triggering receptor expressed on myeloid cells 5;TREM-5;CD300b;CD300LB;CD300B, CLM7, CMRF35A2, IREM3, LMIR5, TREM5;UNQ2530/PRO6029; CD300LB CD300b, CLM-7, CLM7, CMRF35-A2, IREM-3, IREM3, TREM-5, TREM5 CD300 molecule like family member b CMRF35-like molecule 7|CD300 like family member B|immune receptor expressed on myeloid cells 3|leukocyte mono-Ig-like receptor 5|triggering receptor expressed on myeloid cells 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD300LB, check out the CD300LB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD300LB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD300b CD300LB Antibody (A10132)
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Customer Q&As
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5 Customer Q&As for Anti-CD300b CD300LB Antibody
Question
Can you help my question with product A10132, anti-CD300b antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10132 anti-CD300b antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-CD300b antibody A10132 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-CD300b antibody for IHC for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
D. Jha
Verified customer
Asked: 2019-03-07
Answer
As indicated on the product datasheet, A10132 anti-CD300b antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-07
Question
I would like to test anti-CD300b antibody A10132 on human leukocyte for research purposes, then I may be interested in using anti-CD300b antibody A10132 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
The products we sell, including anti-CD300b antibody A10132, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-24
Question
We are currently using anti-CD300b antibody A10132 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
The anti-CD300b antibody (A10132) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-17
