Click image to see more details
Product Info Summary
| SKU: | MA1015 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD3 CD3E Antibody (Monoclonal, CA-3)
SKU/Catalog Number
MA1015
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, CA-3) catalog # MA1015. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD3 CD3E Antibody (Monoclonal, CA-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1015)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
CA-3
Isotype
Mouse IgG1
Immunogen
Human thymocytes followed by Sezary T cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1015 is reactive to CD3E in Human
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
21312 MW
Background of CD3E
The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1015 is guaranteed for Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Positive Control
FCM: human PBMC cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD3 antibody (MA1015).
Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD3 Antibody (MA1015,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse gG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CD3E (Source: Uniprot.org, NCBI)
Gene Name
CD3E
Full Name
T-cell surface glycoprotein CD3 epsilon chain
Weight
21312 MW
Alternative Names
CD3 antigen, epsilon polypeptide (Predicted) ;Protein Cd3e ;Cd3e ;Cd3e_predicted ;rCG_58471 ; CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e , epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell receptor complex, epsilon subunit of T3|T-cell surface T3/Leu-4 epsilon chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD3E, check out the CD3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD3 CD3E Antibody (Monoclonal, CA-3) (MA1015)
Hello CJ!
MA1015 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.
Yu X, Li L, Li Q, Zang X, Liu Z. Biol Trace Elem Res. 2011 Nov;143(2):1064-76. Doi: 10.1007/S12011-010-8941-5. Epub 2011 Jan 12. Trail And Dr5 Promote Thyroid Follicular Cell Apoptosis In Iodine Excess-Induced Experimental Autoimmune Thyroiditis I...
Zhang R, Zhou Sj, Li Cj, Wang Xn, Tang Yz, Chen R, Lv L, Zhao Q, Xing Ql, Yu Dm, Yu P. Lipids Health Dis. 2013 Mar 26;12:42. Doi: 10.1186/1476-511X-12-42. C-Reactive Protein/Oxidised Low-Density Lipoprotein/??2-Glycoprotein I Complex Promotes Athe...
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
Li Y, Geng S, Du X, Chen S, Yang L, Wu X, Li B, Schmidt Ca, Przybylski Gk. Hematology. 2011 Jan;16(1):43-9. Doi: 10.1179/102453311X12902908411634. Restricted Trbv Repertoire In Cd4+ And Cd8+ T-Cell Subsets From Cml Patients.
Liu Yz, Cao Yg, Ye Jq, Wang Wg, Song Kj, Wang Xl, Wang Ch, Li Rt, Deng Xm. Fitoterapia. 2010 Mar;81(2):108-14. Doi: 10.1016/J.Fitote.2009.08.005. Epub 2009 Aug 15. Immunomodulatory Effects Of Proanthocyanidin A-1 Derived In Vitro From Rhododendron...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD3 CD3E Antibody (Monoclonal, CA-3)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD3 CD3E Antibody (Monoclonal, CA-3)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
18 Customer Q&As for Anti-CD3 CD3E Antibody (Monoclonal, CA-3)
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD3 antibody (Monoclonal, CA-3) MA1015. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-20
Question
We are currently using anti-CD3 antibody (Monoclonal, CA-3) MA1015 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The anti-CD3 antibody (Monoclonal, CA-3) (MA1015) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-25
Question
I was wanting to use your anti-CD3 antibody (Monoclonal, CA-3) for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
You can see on the product datasheet, MA1015 anti-CD3 antibody (Monoclonal, CA-3) has been validated for Flow Cytometry, IHC, ICC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
I am looking for to test anti-CD3 antibody (Monoclonal, CA-3) MA1015 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD3 antibody (Monoclonal, CA-3) MA1015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The products we sell, including anti-CD3 antibody (Monoclonal, CA-3) MA1015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-14
Question
Does anti-CD3 antibody (Monoclonal, CA-3) MA1015 work on zebrafish Flow Cytometry with blood?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
Our lab technicians have not tested anti-CD3 antibody (Monoclonal, CA-3) MA1015 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD3 antibody (Monoclonal, CA-3) MA1015 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish blood in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-09
Question
Is a blocking peptide available for product anti-CD3 antibody (Monoclonal, CA-3) (MA1015)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We do provide the blocking peptide for product anti-CD3 antibody (Monoclonal, CA-3) (MA1015). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-06
Question
I see that the anti-CD3 antibody (Monoclonal, CA-3) MA1015 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-19
Question
Do you have a BSA free version of anti-CD3 antibody (Monoclonal, CA-3) MA1015 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD3 antibody (Monoclonal, CA-3) MA1015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
Question
Would anti-CD3 antibody (Monoclonal, CA-3) MA1015 work for Flow Cytometry with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
According to the expression profile of leukemic t-cell, CD3E is highly expressed in leukemic t-cell. So, it is likely that anti-CD3 antibody (Monoclonal, CA-3) MA1015 will work for Flow Cytometry with leukemic t-cell.
Boster Scientific Support
Answered: 2019-06-13
Question
Does MA1015 anti-CD3 antibody (Monoclonal, CA-3) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
You can see on the product datasheet, MA1015 anti-CD3 antibody (Monoclonal, CA-3) as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-24
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-CD3 antibody (Monoclonal, CA-3) MA1015. Please let me know if you require anything else.
L. Johnson
Verified customer
Asked: 2018-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-17
Question
We ordered your anti-CD3 antibody (Monoclonal, CA-3) for ICC on blood a few years ago. I am using human, and We intend to use the antibody for Flow Cytometry next. I was wanting to use examining blood as well as leukemic t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?
A. Parker
Verified customer
Asked: 2018-05-22
Answer
I looked at the website and datasheets of our anti-CD3 antibody (Monoclonal, CA-3) and it seems that MA1015 has been validated on human in both ICC and Flow Cytometry. Thus MA1015 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-22
Question
My question regarding product MA1015, anti-CD3 antibody (Monoclonal, CA-3). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1015 anti-CD3 antibody (Monoclonal, CA-3), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-21
Question
We were content with the WB result of your anti-CD3 antibody (Monoclonal, CA-3). However we have been able to see positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is CD3E supposed to be expressed?
C. Roberts
Verified customer
Asked: 2016-09-22
Answer
From what I have seen in literature, blood does express CD3E. Generally CD3E expresses in cell membrane. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-09-22
Question
What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
Verified Customer
Verified customer
Asked: 2016-09-22
Answer
The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Boster Scientific Support
Answered: 2016-09-22
Question
We are interested in using your anti-CD3 antibody (Monoclonal, CA-3) for t cell receptor signaling pathway studies. Has this antibody been tested with western blotting on human pbmc cells? We would like to see some validation images before ordering.
A. Zhao
Verified customer
Asked: 2016-09-15
Answer
We appreciate your inquiry. This MA1015 anti-CD3 antibody (Monoclonal, CA-3) is tested on human pbmc cells. It is guaranteed to work for Flow Cytometry, IHC, ICC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-09-15
Question
Is this MA1015 anti-CD3 antibody (Monoclonal, CA-3) reactive to the isotypes of CD3E?
P. Jha
Verified customer
Asked: 2015-04-28
Answer
The immunogen of MA1015 anti-CD3 antibody (Monoclonal, CA-3) is Human thymocytes followed by Sezary T cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-04-28
Question
We have observed staining in human leukocyte. What should we do? Is anti-CD3 antibody (Monoclonal, CA-3) supposed to stain leukocyte positively?
R. Kulkarni
Verified customer
Asked: 2015-03-11
Answer
Based on literature leukocyte does express CD3E. Based on Uniprot.org, CD3E is expressed in leukocyte, blood, thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-03-11
