Click image to see more details
Product Info Summary
| SKU: | M03780-5 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD20 Antibody Picoband® (monoclonal, 4D11)
SKU/Catalog Number
M03780-5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD20 Antibody Picoband® (monoclonal, 4D11) catalog # M03780-5. Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD20 Antibody Picoband® (monoclonal, 4D11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03780-5)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
4D11
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03780-5 is reactive to MS4A1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
33.077kDa
Background of CD20
CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What’s more, this protein may be involved in the regulation of B-cell activation and proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03780-5 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human Jurkat whole cell, human Raji whole cell
IHC: human gastric carcinoma tissue, human lienal rupture tissue
IF: human tonsil tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD20 using anti-CD20 antibody (M03780-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD20 antigen affinity purified monoclonal antibody (Catalog # M03780-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD20 at approximately 33 kDa. The expected band size for CD20 is at 33 kDa.
Click image to see more details
Figure 2. IHC analysis of CD20 using anti-CD20 antibody (M03780-5).
CD20 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD20 Antibody (M03780-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD20 using anti-CD20 antibody (M03780-5).
CD20 was detected in a paraffin-embedded section of human lienal rupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD20 Antibody (M03780-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of CD3E and CD20 using anti-CD3E antibody (PB9093) and anti-CD20 antibody (M03780-5).
CD3E and CD20 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD3E antibody (PB9093) and mouse anti-CD20 antibody (M03780-5) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135), DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For MS4A1 (Source: Uniprot.org, NCBI)
Gene Name
MS4A1
Full Name
B-lymphocyte antigen CD20
Weight
33.077kDa
Superfamily
MS4A family
Alternative Names
Eukaryotic translation initiation factor 6; eIF-6; B (2)GCN homolog; B4 integrin interactor; CAB; p27 (BBP); EIF6; EIF3A; ITGB4BP; OK/SW-cl.27 MS4A1 B1, Bp35, CD20, CVID5, FMC7, LEU-16, MS4A2, S7 membrane spanning 4-domains A1 B-lymphocyte CD20|B-lymphocyte cell-surface B1|CD20 |CD20 receptor|leukocyte surface Leu-16|membrane-spanning 4-domains, subfamily A, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MS4A1, check out the MS4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MS4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD20 Antibody Picoband® (monoclonal, 4D11) (M03780-5)
Hello CJ!
No publications found for M03780-5
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD20 Antibody Picoband® (monoclonal, 4D11)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD20 Antibody Picoband® (monoclonal, 4D11)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
