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Product Info Summary
| SKU: | FC00570-mFluor450 |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD2 Monoclonal Antibody mFluor450 Conjugated, Flow Validated
SKU/Catalog Number
FC00570-mFluor450
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD2 Monoclonal Antibody mFluor450 Conjugated, Flow Validated (Catalog# FC00570-mFluor450). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD2 Monoclonal Antibody mFluor450 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00570-mFluor450)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: OKT11
Isotype
IgG1,k
Immunogen
Human acute lymphoblastic leukemia cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00570-mFluor450 is reactive to CD2 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
39448 MW
Background of CD2
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00570-mFluor450 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated blood (RBC lysed) stained with mFluor450 conjugated anti-human CD2 (clone OKT11, red histogram). Lymphocytes gated blood (RBC lysed) stained with FL-6 FMO control (blue histogram). The data were generated in Beckman Coulter CytoFLEX S flowcytometer and analyzed in FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For CD2 (Source: Uniprot.org, NCBI)
Gene Name
CD2
Full Name
T-cell surface antigen CD2
Weight
39448 MW
Alternative Names
T-cell surface antigen CD2;Erythrocyte receptor;LFA-2;LFA-3 receptor;Rosette receptor;T-cell surface antigen T11/Leu-5;CD2;CD2;SRBC; CD2 LFA-2, SRBC, T11 CD2 molecule T-cell surface CD2|CD2 (p50), sheep red blood cell receptor|LFA-3 receptor|T-cell surface T11/Leu-5|erythrocyte receptor|lymphocyte-function -2|rosette receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD2, check out the CD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD2 Monoclonal Antibody mFluor450 Conjugated, Flow Validated (FC00570-mFluor450)
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No publications found for FC00570-mFluor450
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Customer Q&As
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3 Customer Q&As for Anti-Human CD2 Monoclonal Antibody mFluor450 Conjugated, Flow Validated
Question
I am interested in to test anti-Human CD2 Monoclonal antibody mFluor450 Conjugated, Flow Validated FC00570-mFluor450 on human leukocyte for research purposes, then I may be interested in using anti-Human CD2 Monoclonal antibody mFluor450 Conjugated, Flow Validated FC00570-mFluor450 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The products we sell, including anti-Human CD2 Monoclonal antibody mFluor450 Conjugated, Flow Validated FC00570-mFluor450, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-12
Question
I was wanting to use your anti-Human CD2 Monoclonal antibody mFluor450 Conjugated, Flow Validated for Flow Cytometry for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
You can see on the product datasheet, FC00570-mFluor450 anti-Human CD2 Monoclonal antibody mFluor450 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-07
Question
Is this FC00570-mFluor450 anti-Human CD2 Monoclonal antibody mFluor450 Conjugated, Flow Validated reactive to the isotypes of CD2?
D. Huang
Verified customer
Asked: 2017-02-21
Answer
The immunogen of FC00570-mFluor450 anti-Human CD2 Monoclonal antibody mFluor450 Conjugated, Flow Validated is Human acute lymphoblastic leukemia cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-02-21
