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Product Info Summary
| SKU: | A03958 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD177 antigen CD177 Antibody
SKU/Catalog Number
A03958
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD177 antigen CD177 Antibody catalog # A03958. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD177 antigen CD177 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03958)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD177. AA range:361-410
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03958 is reactive to CD177 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
46363 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03958 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using CD177 Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Colon, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For CD177 (Source: Uniprot.org, NCBI)
Gene Name
CD177
Full Name
CD177 antigen
Weight
46363 MW
Alternative Names
CD177 antigen;Human neutrophil alloantigen 2a;HNA-2a;NB1 glycoprotein;NB1 GP;Polycythemia rubra vera protein 1;PRV-1;CD177;CD177;NB1, PRV1;UNQ595/PRO1181; CD177 HNA-2a, HNA2A, NB1, NB1 GP, PRV-1, PRV1 CD177 molecule CD177 |NB1 glycoprotein|cell surface receptor|human neutrophil allo 2a|polycythemia rubra vera protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD177, check out the CD177 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD177: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD177 antigen CD177 Antibody (A03958)
Hello CJ!
A03958 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chitosan nanoparticles attenuate intestinal damage and inflammatory responses in LPS-challenged weaned piglets via prevention of IκB degradation
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Customer Q&As
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4 Customer Q&As for Anti-CD177 antigen CD177 Antibody
Question
Would anti-CD177 antibody A03958 work on pig WB with blood?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Our lab technicians have not tested anti-CD177 antibody A03958 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CD177 antibody A03958 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-20
Question
I see that the anti-CD177 antibody A03958 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-11
Question
Is there a BSA free version of anti-CD177 antibody A03958 available?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD177 antibody A03958 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-CD177 antibody A03958 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-CD177 antibody (A03958) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
