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Anti-CD166 Monoclonal Antibody

ALCAM/CD166 antibody

Boster Bio Anti-CD166 Monoclonal Antibody catalog # M01788. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01788
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-CD166 Monoclonal Antibody

View all ALCAM/CD166 Antibodies

SKU/Catalog Number

M01788
BM5440 is an alternative SKU for this antibody, used in previous lots.

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD166 Monoclonal Antibody catalog # M01788. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD166 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01788)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADGA-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD166

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01788 is reactive to ALCAM in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

40 kDa

Calculated molecular weight

93558 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01788 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:60

Positive Control

WB: Daudi cell

Validation Images & Assay Conditions

Gene/Protein Information For ALCAM (Source: Uniprot.org, NCBI)

Gene Name

ALCAM

Full Name

CD166 antigen

Weight

93558 MW

Alternative Names

Axin-2;Axin-like protein;Axil;Axis inhibition protein 2;Conductin;AXIN2; ALCAM CD166, MEMD activated leukocyte cell adhesion molecule CD166

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALCAM, check out the ALCAM Infographic

ALCAM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-CD166 Monoclonal Antibody

Question

Will anti-CD166 Monoclonal antibody M01788 work for IF with lung placenta?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

According to the expression profile of lung placenta, ALCAM is highly expressed in lung placenta. So, it is likely that anti-CD166 Monoclonal antibody M01788 will work for IF with lung placenta.

Boster Scientific Support

Answered: 2020-04-27

Question

Does anti-CD166 Monoclonal antibody M01788 work on feline IP with plasma?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

Our lab technicians have not validated anti-CD166 Monoclonal antibody M01788 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CD166 Monoclonal antibody M01788 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline plasma in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-27

Question

Would M01788 anti-CD166 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

V. Lewis

Verified customer

Asked: 2014-04-16

Answer

It shows on the product datasheet, M01788 anti-CD166 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-04-16

Order DetailsPrice
M01788

100μL

$370
M01788-30ul

30μL sample

$99
M01788-carrier-free-1ml

Carrier Free

$2590

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M01788
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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