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Product Info Summary
| SKU: | M01788 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD166 Monoclonal Antibody
View all ALCAM/CD166 Antibodies
SKU/Catalog Number
M01788
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD166 Monoclonal Antibody catalog # M01788. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD166 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01788)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADGA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD166
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01788 is reactive to ALCAM in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
93558 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01788 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:60
Positive Control
WB: Daudi cell
Validation Images & Assay Conditions
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Western blot analysis of CD166 expression in Daudi cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ALCAM (Source: Uniprot.org, NCBI)
Gene Name
ALCAM
Full Name
CD166 antigen
Weight
93558 MW
Alternative Names
Axin-2;Axin-like protein;Axil;Axis inhibition protein 2;Conductin;AXIN2; ALCAM CD166, MEMD activated leukocyte cell adhesion molecule CD166
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALCAM, check out the ALCAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD166 Monoclonal Antibody (M01788)
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Customer Q&As
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3 Customer Q&As for Anti-CD166 Monoclonal Antibody
Question
Will anti-CD166 Monoclonal antibody M01788 work for IF with lung placenta?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
According to the expression profile of lung placenta, ALCAM is highly expressed in lung placenta. So, it is likely that anti-CD166 Monoclonal antibody M01788 will work for IF with lung placenta.
Boster Scientific Support
Answered: 2020-04-27
Question
Does anti-CD166 Monoclonal antibody M01788 work on feline IP with plasma?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Our lab technicians have not validated anti-CD166 Monoclonal antibody M01788 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CD166 Monoclonal antibody M01788 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline plasma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
Would M01788 anti-CD166 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Lewis
Verified customer
Asked: 2014-04-16
Answer
It shows on the product datasheet, M01788 anti-CD166 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-04-16
