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Product Info Summary
| SKU: | A07091-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-CD103/Itgae Antibody
SKU/Catalog Number
A07091-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD103/Itgae Antibody catalog # A07091-2. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD103/Itgae Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07091-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD103/Itgae recombinant protein (Position: F20-E1061).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07091-2 is reactive to Itgae in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
81 kDa
Calculated molecular weight
130.159kDa
Background of ITGAE
ITGAE(INTEGRIN, ALPHA-E), also known as CD103, is an integrin protein that in human is encoded by the ITGAE gene. The ITGAE gene is mapped to chromosome 17p13 by inclusion within a BAC contig. ITGAE is expressed widely on intraepithelial lymphocyte(IEL) T cells(both alphabeta T cells and gammadelta T cells) and on some peripheral regulatory T cells(Tregs) which is important for decreasing the immune response and appears to play a crucial role in the prevention of autoimmune diseases. ITGAE shares overall homology with other alpha integrins but has a unique 55-amino acid "extra" region located just N-terminal to the I domain that contains 18 consecutive charged residues and a proteolytic cleavage site.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07091-2 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: mouse colon tissue, rat colon tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (A07091-2).
CD103/Itgae was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD103/Itgae Antibody (A07091-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (A07091-2).
CD103/Itgae was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD103/Itgae Antibody (A07091-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Itgae (Source: Uniprot.org, NCBI)
Gene Name
Itgae
Full Name
Integrin alpha-E
Weight
130.159kDa
Superfamily
integrin alpha chain family
Alternative Names
Ribosome-binding protein 1; 180 kDa ribosome receptor homolog; RRp; ES/130-related protein; Ribosome receptor protein; RRBP1; KIAA1398 ITGAE CD103, HUMINAE integrin subunit alpha E integrin alpha-E|HML-1 | CD103, human mucosal lymphocyte 1; alpha polypeptide|human mucosal lymphocyte 1, alpha polypeptide|integrin alpha-IEL|integrin, alpha E ( CD103, human mucosal lymphocyte 1; alpha polypeptide)|mucosal lymphocyte 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Itgae, check out the Itgae Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Itgae: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD103/Itgae Antibody (A07091-2)
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