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Product Info Summary
| SKU: | A07106-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-CD101 Antibody
View all IGSF2/CD101 Antibodies
SKU/Catalog Number
A07106-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD101 Antibody catalog # A07106-1. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07106-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD101 recombinant protein (Position: E53-A904).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07106-1 is reactive to CD101 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
124 kDa
Calculated molecular weight
115.109kDa
Background of IGSF2/CD101
Immunoglobulin superfamily, member 2 (IGSF2) also known as CD101 (Cluster of Differentiation 101), is a human gene. Predicted to enable hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides. Predicted to be involved in cell surface receptor signaling pathway. Predicted to act upstream of or within positive regulation of myeloid leukocyte differentiation. Located in extracellular exosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07106-1 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: human adenocarcinoma of the right colon tissue, human liver cancer tissue, human lung cancer tissue, human placenta tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of CD101 using anti-CD101 antibody (A07106-1).
CD101 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of CD101 using anti-CD101 antibody (A07106-1).
CD101 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD101 using anti-CD101 antibody (A07106-1).
CD101 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CD101 using anti-CD101 antibody (A07106-1).
CD101 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD101 (Source: Uniprot.org, NCBI)
Gene Name
CD101
Full Name
Immunoglobulin superfamily member 2
Weight
115.109kDa
Alternative Names
Ribosome-binding protein 1; 180 kDa ribosome receptor homolog; RRp; ES/130-related protein; Ribosome receptor protein; RRBP1; KIAA1398 CD101 EWI-101, IGSF2, V7 CD101 molecule immunoglobulin superfamily member 2|cell surface glycoprotein V7|glu-Trp-Ile EWI motif-containing protein 101|leukocyte surface protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD101, check out the CD101 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD101: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD101 Antibody (A07106-1)
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