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Product Info Summary
| SKU: | PB9549 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CCN1/CYR61 Antibody Picoband®
View all Cyr61/CCN1 Antibodies
SKU/Catalog Number
PB9549
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCN1/CYR61 Antibody Picoband® catalog # PB9549. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCN1/CYR61 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9549)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CCN1 recombinant protein (Position: K54-D381). Human CCN1 shares 91.8% and 92.1% amino acid (aa) sequence identity with mouse and rat CCN1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9549 is reactive to CYR61 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42027 MW
Background of Cyr61/CCN1
Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. CYR61 is a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family (CCN intercellular signaling protein). And it is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9549 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Thymus Tissue, Rat Skeletal Muscle Tissue, A431 Whole Cell, SGC Whole Cell, 22RV1 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CCN1 using anti-CCN1 antibody (PB9549).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Thymus Tissue Lysate at 50ug,
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug,
Lane 3: A431 Whole Cell Lysate at 40ug,
Lane 4: SGC Whole Cell Lysate at 40ug,
Lane 5: 22RV1 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN1 antigen affinity purified polyclonal antibody (Catalog # PB9549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCN1 at approximately 42 kDa. The expected band size for CCN1 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CYR61 (Source: Uniprot.org, NCBI)
Gene Name
CYR61
Full Name
CCN family member 1
Weight
42027 MW
Superfamily
CCN family
Alternative Names
Protein CYR61;CCN family member 1;Cysteine-rich angiogenic inducer 61;Insulin-like growth factor-binding protein 10;IBP-10;IGF-binding protein 10;IGFBP-10;Protein GIG1;CYR61;CCN1, GIG1, IGFBP10; CCN1 CYR61, GIG1, IGFBP10 cellular communication network factor 1 CCN family member 1|IBP-10|IGF-binding protein 10|IGFBP-10|cysteine rich angiogenic inducer 61|cysteine-rich heparin-binding protein 61|cysteine-rich, anigogenic inducer, 61|insulin-like growth factor-binding protein 10|protein CYR61
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYR61, check out the CYR61 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYR61: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCN1/CYR61 Antibody Picoband® (PB9549)
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Customer Q&As
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2 Customer Q&As for Anti-CCN1/CYR61 Antibody Picoband®
Question
Which amino acids or protein structure PB9549 binds to?
Verified customer
Asked: 2021-02-12
Answer
The immunogen sequence for the Anti-CCN1/CYR61 Antibody Picoband™ (PB9549) is K54-D381.
Boster Scientific Support
Answered: 2021-02-16
Question
We are currently using anti-CCN1/CYR61 antibody PB9549 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on goat tissues as well?
M. Rodriguez
Verified customer
Asked: 2013-08-02
Answer
The anti-CCN1/CYR61 antibody (PB9549) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-02
