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Product Info Summary
| SKU: | M01370 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-CYP27B1 Antibody (C-term)
SKU/Catalog Number
M01370
Size
50 µl
Description
Boster Bio Anti-CYP27B1 Antibody (C-term) (Catalog # M01370). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CYP27B1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01370)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1981CT820.303.81
Isotype
IgG1,κ
Immunogen
This CYP27B1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 477-508 amino acids from the C-terminal region of human CYP27B1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01370 is reactive to CYP27B1 in Human
Reconstitution
Calculated molecular weight
56504 Da
Background of CYP27B1
Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01370 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Anti-CYP27B1 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For CYP27B1 (Source: Uniprot.org, NCBI)
Gene Name
CYP27B1
Full Name
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Weight
56504 Da
Superfamily
cytochrome P450 family
Alternative Names
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 1.14.13.13, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B CYP27B1 CP2B, CYP1, CYP1alpha, CYP27B, P450c1, PDDR, VDD1, VDDR, VDDRI, VDR cytochrome P450 family 27 subfamily B member 1 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial|1alpha(OH)ase|25 hydroxyvitamin D3-1-alpha hydroxylase|25-OHD-1 alpha-hydroxylase|VD3 1A hydroxylase|calcidiol 1-monooxygenase|cytochrome P450 subfamily XXVIIB polypeptide 1|cytochrome P450, family 27, subfamily B, polypeptide 1|cytochrome P450C1 alpha|cytochrome P450VD1-alpha|cytochrome p450 27B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP27B1, check out the CYP27B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP27B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP27B1 Antibody (C-term) (M01370)
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