Anti-CBS Antibody Picoband® (monoclonal, 7C3B7)

Product Name

Anti-CBS Antibody Picoband® (monoclonal, 7C3B7)

View all CBS Antibodies

SKU/Catalog Number

M00130-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CBS Antibody Picoband® (monoclonal, 7C3B7) catalog # M00130-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CBS Antibody Picoband® (monoclonal, 7C3B7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00130-2)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

7C3B7

Isotype

Mouse IgG2b

Standard Protein

E.coli-derived human CBS recombinant protein (Position: K102-E342).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00130-2 is reactive to CBS in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

61 kDa

Calculated molecular weight

46659 MW

Background of CBS

CBS, Cystathionine Beta-Synthase, catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity. The CBS gene, which is mapped to chromosome 21q22, contains 23 exons, ranging in size from 42 to 299 bp. The human CBS protein can substitute for the endogenous yeast CBS protein in Saccharomyces cerevisiae. The catalytic domain of the CBS protein is located in the N-terminal 409 amino acids, and a regulatory domain is located in the C-terminal 142 amino acids.A mutation that deletes the C-terminal 145 amino acids of CBS could restore activity of several CBS mutant alleles found in homocystinurics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00130-2 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Positive Control

WB: human Hela whole cell, human 293T whole cell, human K562 whole cell, rat liver tissue, rat pancreas tissue, mouse liver tissue, mouse pancreas tissue

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CBS using anti-CBS antibody (M00130-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CBS antigen affinity purified monoclonal antibody (Catalog # M00130-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CBS at approximately 61 kDa. The expected band size for CBS is at 61 kDa.

Gene/Protein Information For CBS (Source: Uniprot.org, NCBI)

Alternative Names

cystathionine-beta-synthase; EC 4.2.1.2210Beta-thionase CBS CBSL, HIP4 cystathionine beta-synthase cystathionine beta-synthase|Cystathionine beta-synthase-like protein|beta-thionase|methylcysteine synthase|serine sulfhydrase

For more info on CBS, check out the CBS Infographic

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In this infographic, you will see the following information for CBS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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