CBS Antibodies

Cystathionine beta-synthase (CBS)

Hydro-lyase catalyzing the initial step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine at a beta-replacement response to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine as well as the toxic metabolite L- homocysteine (PubMed:23981774, PubMed:20506325, PubMed:23974653).

Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity). .

Gene Information

Human
Gene Name:	CBS
Uniprot:	P35520
Entrez:	875

Family

Belongs to:
cysteine synthase/cystathionine beta-synthase family

Alternative Names

cystathionine-beta-synthase; EC 4.2.1.2210Beta-thionase

Molecular Weight

Mass (kDA):
60.587 kDA

Genomic Context

Human
Location:	21q22.3
Sequence:	21; NC_000021.9 (43053190..43076861, complement)

Tissue Specificity

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

Subcellular Localizations

Cytoplasm. Nucleus.

Diseases

• Hyperhomocysteinemia
• 5,10-methylenetetrahydrofolate Reductase Deficiency
• Homocystinuria
• Cystathionine Beta-synthase Deficiency Disease
• Malignant Neoplasms
• Ulcer
• Cardiovascular Diseases
• Cerebrovascular Accident
• Thrombosis
• Neoplasms
• Infection By Cryptococcus Neoformans
• Duodenal Ulcer
• Dementia

• Vascular Diseases
• Alzheimer's Disease
• Carcinoma
• Helicobacter Infections
• Atrophy
• Inflammation
• Neurodegenerative Disorders

Interacting Proteins

• EHHADH
• PIN1
• PRKAG1
• PSMA1
• UBASH3A

Pathways

• Fermentation
• Transsulfuration
• Transport
• Pathogenesis
• Methylation
• Secretion
• Mating
• Localization
• Cell Growth
• Excretion
• Dna Methylation
• Cell Cycle
• Cell Death

• Translation
• Vasodilation
• Aging
• Hemostasis
• Glycosylation
• Chromosome Breakage
• Cognition

PTMs

• Methylation
• Phosphorylation
• Cleavage
• Reduction
• Oxidation
• Nitration
• Glycosylation
• Hydroxylation
• Acylation
• Sumoylation

• Decarboxylation
• Cysteinylation
• Amination
• Demethylation
• Glutathionylation
• Phosphorothioation
• Deamination
• Dephosphorylation
• C-mannosylation
• Palmitoylation

Research Areas

• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CBS

Boster Bio: Best Uses of the CBS Marker

Boster Bio Best Uses of the CBS Marker will teach you about the molecular biology methods Boster uses in his research. Techniques of molecular biology are employed to manipulate and study genetic materials. Boster provides a wide range of technical sources, blogs, and information on diseases and digital tools that are accessible via the internet. Furthermore, Boster provides high-quality lysis buffers that guarantee good results and minimize the intensity of cross-linking.

Techniques for molecular biology

Methods of molecular biology are used to detect RNAs and proteins in a variety of biological samples. This involves conjugating secondary antibodies with fluorescent molecules. Fluorophores are fluorescent molecules that are excited by light and emit photons that are detectable. The amount of light produced by the fluorophores is directly proportional to the amount of protein that is bound to the membrane. These techniques allow researchers to study the concentrations of target proteins in different tissues or in samples.

The techniques of molecular biology include the use of caspases which are cysteine proteases that respond to apoptosis stimuli. These triggers could be cell surface or mitochondrial. Pro-apoptotic proteins include tumor necrosis factor (TNF), FasL (growth factor receptor-bound protein) Granzyme B (DNA damage), and calcium channels. Molecular biology techniques used in the research of cancer cells are highly varied and Boster provides complete reagents and practical solutions for PCR.

PCR is a common method for detecting expression of genes. The PCR procedure involves amplifying a particular DNA sequence, or RNA sequence which is usually a gene. The strands of DNA are linked with a primer and SYBR green dye. A fluorescence analysis is employed to identify the PCR product when these two strands join. This method also allows researchers to determine the DNA content of an individual sample.

The PCR technique is an extremely sensitive method of DNA analysis, allowing scientists to identify viruses early. Viral DNA can be easily detected through the PCR method. Primers should be specific to specific sequences. This high sensitivity makes PCR an essential instrument to detect viruses early in an infection cycle and provide doctors with an advantage in the treatment process. The PCR technique has also been vital in determining the presence of genetically rare diseases.

Western blotting is a semi-quantitative method for finding proteins. Proteins are distinguished by their molecular weight. Then, they are transferred to a solid membrane, where a secondary antibody binds to the target protein. These proteins are then detected by various detection systems. Blotting is extremely complex and requires expertise. The results of these tests depend on many factors.

DNA library technology is a fundamental method in molecular biology. DNA libraries can be used to store different types of genetic material, ranging from cDNA to mitochondrial DNA. A DNA library can store various types of genetic material such as cDNA, mitochondrial and reverse-transcribed DNA. Other genetic elements can be stored in DNA libraries, including RNA and mitochondria.

Another technique in cell biology is flow cytometry. This technique makes use of laser-based technology. This technique employs a single-file laser beam to measure fluorescence intensity. The intensity of the fluorescence corresponds to the amount of a cellular component. This technique is typically employed to determine the phenotype and the function of a biological sample. One of the advantages of flow cytometry is its high-throughput capacity. It is also able to analyze many samples simultaneously.

The synthesis of RNA can be a complex process. There are a variety of RNA types involved in the process of synthesis of proteins. Messenger RNA transmits genetic data from DNA to the ribosome. RibosomalRNA converts amino acids to proteins. The replication of DNA is accomplished by the polymerase enzyme that is found in cells. The enzyme RNA Polymerase works on DNA to create copies.

Steven Boster uses molecular biology techniques

Students studying molecular Biology learn how DNA operates and how it can be used to alter gene expression. They also learn how chemical energy is used to power a variety of biological processes. They also study the mitochondria and chloroplasts two organelles that regulate the chemistry of photosynthesis and the process of respiration in cells. In addition to basic principles students will also learn about the process of cellular communication that describe how cell-to cell chemical signals are communicated and how the environment of the cell affects protein activity.

Students studying molecular science may be able to use new technologies to conduct research on DNA repair. Researchers have been able to observe a protein involved DNA repair as well as other intricate processes. These new approaches to medicine may result in targeted therapies which block cancer cells from self-repairing. Undergraduate students are creating DNA-based diagnostic tools that utilize DNA to determine if mosquitoes carry Zika virus or other bloodborne illnesses. Weight loss programs specific to the individual's genetic profile are also in the works.