Product Info Summary
SKU: | PB9166 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Caveolin-2/CAV2 Antibody Picoband™
View all Caveolin-2 Antibodies
SKU/Catalog Number
PB9166
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caveolin-2/CAV2 Antibody Picoband™ catalog # PB9166. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caveolin-2/CAV2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9166)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Caveolin-2 recombinant protein (Position: M1-D162). Human Caveolin-2 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat Caveolin-2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9166 is reactive to CAV2 in Human, Mouse, Rat
Applications
PB9166 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
21 kDa
Calculated molecular weight
18291 MW
Background of Caveolin-2
Caveolin-2 is a protein that in humans is encoded by the CAV2 gene. It is mapped to 7q31.1-q31.2. The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. Caveolin-2 is a protein related to caveolin-1 which is derived caveolin-enriched membranes. CAV2 and CAV1 are similar in most respects and they differ in their functional interactions with heterotrimeric G proteins. Both of them are expressed in neuronal cells. Caveolin-2 was upregulated in response to neuronal cell injury.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunofluorescence, 2μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
![pb9166 caveolin 2 primary antibodies wb testing 1 pb9166 caveolin 2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/p/b/pb9166-caveolin-2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HT1080 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-2 antigen affinity purified polyclonal antibody (Catalog # PB9166) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-2 at approximately 21KD. The expected band size for Caveolin-2 is at 21KD.
![pb9166 caveolin 2 primary antibodies ihc testing 2 pb9166 caveolin 2 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/p/b/pb9166-caveolin-2-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166).
Caveolin-2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9166 caveolin 2 primary antibodies ihc testing 3 pb9166 caveolin 2 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/p/b/pb9166-caveolin-2-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166).
Caveolin-2 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![pb9166 caveolin 2 primary antibodies if testing 4 pb9166 caveolin 2 primary antibodies if testing 4](https://www.bosterbio.com/media/catalog/product/p/b/pb9166-caveolin-2-primary-antibodies-if-testing-4.jpg)
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Figure 4. IF analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166).
Caveolin-2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![pb9166 caveolin 2 primary antibodies if testing 5 pb9166 caveolin 2 primary antibodies if testing 5](https://www.bosterbio.com/media/catalog/product/p/b/pb9166-caveolin-2-primary-antibodies-if-testing-5.jpg)
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Figure 5. IF analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166).
Caveolin-2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![pb9166 caveolin 2 primary antibodies fcm testing 6 pb9166 caveolin 2 primary antibodies fcm testing 6](https://www.bosterbio.com/media/catalog/product/p/b/pb9166-caveolin-2-primary-antibodies-fcm-testing-6.jpg)
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Figure 6. Flow Cytometry analysis of A549 cells using anti-Caveolin-2 antibody (PB9166).
Overlay histogram showing A549 cells stained with PB9166 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-2 Antibody (PB9166,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CAV2 (Source: Uniprot.org, NCBI)
Gene Name
CAV2
Full Name
Caveolin-2
Weight
18291 MW
Superfamily
caveolin family
Alternative Names
CAV; CAV2; caveolae protein, 20-kD; caveolin 2 isoform a and b; caveolin 2; Caveolin2; Caveolin-2; MGC12294 CAV2 CAV caveolin 2 caveolin-2|caveolae protein, 20-kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAV2, check out the CAV2 Infographic
![CAV2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAV2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caveolin-2/CAV2 Antibody Picoband™ (PB9166)
Hello CJ!
PB9166 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang Nn, Zhao Lj, Wu Ln, He Mf, Qu Jw, Zhao Yb, Zhao Wz, Li Js, Wang Jh. Asian Pac J Cancer Prev. 2013;14(9):4983-8. Mechanistic Analysis Of Taxol-Induced Multidrug Resistance In An Ovarian Cancer Cell Line.
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Customer Q&As
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6 Customer Q&As for Anti-Caveolin-2/CAV2 Antibody Picoband™
Question
I see that the anti-Caveolin-2/CAV2 antibody PB9166 works with IHC-F, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can find protocols for IHC-F on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-24
Question
My lab would like to test anti-Caveolin-2/CAV2 antibody PB9166 on human hepatoma for research purposes, then I may be interested in using anti-Caveolin-2/CAV2 antibody PB9166 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The products we sell, including anti-Caveolin-2/CAV2 antibody PB9166, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-Caveolin-2/CAV2 antibody PB9166 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
The anti-Caveolin-2/CAV2 antibody (PB9166) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-03
Question
Would anti-Caveolin-2/CAV2 antibody PB9166 work for IHC-F with hepatoma?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
According to the expression profile of hepatoma, CAV2 is highly expressed in hepatoma. So, it is likely that anti-Caveolin-2/CAV2 antibody PB9166 will work for IHC-F with hepatoma.
Boster Scientific Support
Answered: 2018-03-16
Question
Is a blocking peptide available for product anti-Caveolin-2/CAV2 antibody (PB9166)?
B. Zhang
Verified customer
Asked: 2015-05-14
Answer
We do provide the blocking peptide for product anti-Caveolin-2/CAV2 antibody (PB9166). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-05-14
Question
Is there a BSA free version of anti-Caveolin-2/CAV2 antibody PB9166 available?
C. Collins
Verified customer
Asked: 2014-07-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Caveolin-2/CAV2 antibody PB9166 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-07-03