Click image to see more details
Product Info Summary
| SKU: | A00080 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Bovine, Chicken, Dog, Guinea pig, Human, Monkey, Mouse, Rat, Sheep |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Caspase-9 CASP9 Antibody
SKU/Catalog Number
A00080
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-Caspase-9 CASP9 Antibody (Catalog# A00080). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Monkey, Sheep.
Storage & Handling
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Cite This Product
Anti-Caspase-9 CASP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00080)
Host
Rabbit
Contents
Liquid. In PBS containing 0.09% sodium azide.
Clonality
Polyclonal
Immunogen
Recombinant human procaspase-9 (N-terminal fragment)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00080 is reactive to CASP9 in Bovine, Chicken, Dog, Guinea pig, Human, Monkey, Mouse, Rat, Sheep
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
46281 MW
Background of Caspase-9
CASP9 (CASPASE9), also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The CASP9 gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in caspase-3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Its genomic coordinates (GRCh37) is 1:15,818,768-15,851,284. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the CASP9 gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c and dATP, an event that leads to caspase-9 activation. CASP9 activity increases dramatically upon association with the apoptosome complex. And the majority of Casp9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00080 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1:500, ECL). Detects bands of ~46-50kDa (proform) and ~35/37kDa, ~18kDa, ~10kDa (processed) by Western blot.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Caspase-9 expression in untreated Jurkat cell lysates (lane 1), and apoptosisinduced Jurkat cell lysates (lane 2). Caspase-9 at 35/37kD, 18kD, 10kD was detected using rabbit anti-Caspase-9 Antigen Affinity purified polyclonal antibody (Catalog # A00080) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)
Gene Name
CASP9
Full Name
Caspase-9
Weight
46281 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6; CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP9, check out the CASP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-9 CASP9 Antibody (A00080)
Hello CJ!
A00080 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line
In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer
Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.
Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Caspase-9 CASP9 Antibody
Question
Does A00080 anti-Caspase-9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Krishna
Verified customer
Asked: 2020-04-08
Answer
It shows on the product datasheet, A00080 anti-Caspase-9 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-08
Question
Here is the WB image, lot number and protocol we used for t-cell using anti-Caspase-9 antibody A00080. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-10
Question
Is this A00080 anti-Caspase-9 antibody reactive to the isotypes of CASP9?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The immunogen of A00080 anti-Caspase-9 antibody is Recombinant human procaspase-9 (N-terminal fragment). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-04
Question
I am interested in using your anti-Caspase-9 antibody for response to estradiol studies. Has this antibody been tested with western blotting on jurkat cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Thanks for your inquiry. This A00080 anti-Caspase-9 antibody is validated on jurkat cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-20
Question
Is there a BSA free version of anti-Caspase-9 antibody A00080 available?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-9 antibody A00080 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-01
Question
Does anti-Caspase-9 antibody A00080 work for WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
According to the expression profile of t-cell, CASP9 is highly expressed in t-cell. So, it is likely that anti-Caspase-9 antibody A00080 will work for WB with t-cell.
Boster Scientific Support
Answered: 2019-07-15
Question
Is a blocking peptide available for product anti-Caspase-9 antibody (A00080)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
We do provide the blocking peptide for product anti-Caspase-9 antibody (A00080). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-01
Question
I was wanting to use your anti-Caspase-9 antibody for WB for rat t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat t-cell identification?
M. Li
Verified customer
Asked: 2019-03-05
Answer
It shows on the product datasheet, A00080 anti-Caspase-9 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-05
Question
We have been able to see staining in mouse eye lymph. Any tips? Is anti-Caspase-9 antibody supposed to stain eye lymph positively?
K. Yang
Verified customer
Asked: 2018-08-21
Answer
From what I have seen in literature eye lymph does express CASP9. From what I have seen in Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201
Boster Scientific Support
Answered: 2018-08-21
Question
We are currently using anti-Caspase-9 antibody A00080 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
The anti-Caspase-9 antibody (A00080) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-9 antibody A00080. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-19
Question
Can you help my question with product A00080, anti-Caspase-9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Moore
Verified customer
Asked: 2016-11-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00080 anti-Caspase-9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-11-04
Question
I see that the anti-Caspase-9 antibody A00080 works with WB, what is the protocol used to produce the result images on the product page?
F. Miller
Verified customer
Asked: 2013-08-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-08-14
Question
We want to test anti-Caspase-9 antibody A00080 on rat t-cell for research purposes, then I may be interested in using anti-Caspase-9 antibody A00080 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Jones
Verified customer
Asked: 2013-02-14
Answer
The products we sell, including anti-Caspase-9 antibody A00080, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-02-14
