Product Info Summary
SKU: | M00042-4 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-8 CASP8 Monoclonal Antibody
SKU/Catalog Number
M00042-4
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Caspase-8 CASP8 Monoclonal Antibody catalog # M00042-4. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-8 CASP8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00042-4)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
2G12
Isotype
IgG
Immunogen
Recombinant Protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00042-4 is reactive to CASP8 in Human, Mouse, Rat
Applications
M00042-4 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55391 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-2000
IHC1:200-500
Validation Images & Assay Conditions
![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 2 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-2.jpg)
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Figure 2. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemical analysis of human-colon tissue. Anti-Caspase-8 at 1:200 (4°C![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 3 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-3.jpg)
Click image to see more details
Figure 3. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemical analysis of human liver cancer tissue. Anti-Caspase-8 at 1:200 (4°C![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 4 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-4.jpg)
Click image to see more details
Figure 4. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemical analysis of human liver tissue. Anti-Caspase-8 at 1:200 (4°C![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 5 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 5](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-5.jpg)
Click image to see more details
Figure 5. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemical analysis of human lung cancer tissue. Anti-Caspase-8 at 1:200 (4°C![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 6 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 6](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-6.jpg)
Click image to see more details
Figure 6. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemical analysis of human stomach cancer tissue. Anti-Caspase-8 at 1:200 (4°C![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 7 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 7](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-7.jpg)
Click image to see more details
Figure 7. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemical analysis of human tonsil tissue. Anti-Caspase-8 at 1:200 (4°C![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 8 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 8](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-8.jpg)
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Figure 8. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemical analysis of mouse brain tissue. M00042-4 was diluted at 1:200 (4°C![m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 9 m00042 4 anti caspase 8 casp8 monoclonal antibody ihc testing 9](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-ihc-testing-9.jpg)
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Figure 9. Immunohistochemistry validation of CASP8 using Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4).
Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Spleen Tissue using Caspase-8 monoclonal antibody.For more protocol information of IHC
![m00042 4 anti caspase 8 casp8 monoclonal antibody wb testing 1 m00042 4 anti caspase 8 casp8 monoclonal antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m00042-4-anti-caspase-8-casp8-monoclonal-antibody-wb-testing-1.jpg)
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Figure 1. Western blotting validation for Anti-Caspase-8 CASP8 Monoclonal Antibody M00042-4
Western blot (WB) analysis of Caspase-8 monoclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CASP8 (Source: Uniprot.org, NCBI)
Gene Name
CASP8
Full Name
Caspase-8
Weight
55391 MW
Superfamily
peptidase C14A family
Alternative Names
AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8, check out the CASP8 Infographic
![CASP8 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-8 CASP8 Monoclonal Antibody (M00042-4)
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