Product Info Summary
SKU: | PA1961-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-3(P17)/CASP3 Antibody Picoband®
SKU/Catalog Number
PA1961-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-3 (P17)/CASP3 Antibody catalog # PA1961-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-3(P17)/CASP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1961-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17), different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1961-1 is reactive to Casp3 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
32 kDa, 19 kDa
Calculated molecular weight
31475 MW
Background of Caspase-3
CASP3 (Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Tiso et al. (1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al. (1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al. (1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1961-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Heart Tissue, Rat Liver Tissue, Rat Thymus Tissue, Rat Spleen Tissue, mouse thymus tissue, mouse spleen tissue, mouse lung tissue, mouse brain tissue, mouse testis tissue
IHC: rat intestine tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Caspase-3 (P17) using anti-Caspase-3 (P17) antibody (PA1961-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Thymus Tissue Lysate
Lane 4: Rat Spleen Tissue Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 (P17) antigen affinity purified polyclonal antibody (Catalog # PA1961-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 (P17) at approximately 32KD,19KD. The expected band size for Caspase-3 (P17) is at 32KD,17KD.
Click image to see more details
Figure 2. IHC analysis of Caspase-3 (P17)using anti-Caspase-3 (P17)antibody (PA1961-1).
Caspase-3 (P17)was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase-3 (P17)Antibody (PA1961-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of Caspase-3 (P17) using anti-Caspase-3 (P17) antibody (PA1961-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 (P17) antigen affinity purified polyclonal antibody (Catalog # PA1961-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 (P17) at approximately 32KD,19KD. The expected band size for Caspase-3 (P17) is at 32KD,17KD.
Protein Target Info & Infographic
Gene/Protein Information For Casp3 (Source: Uniprot.org, NCBI)
Gene Name
Casp3
Full Name
Caspase-3
Weight
31475 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;LICE;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;Casp3;Cpp32; CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Casp3, check out the Casp3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Casp3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-3(P17)/CASP3 Antibody Picoband® (PA1961-1)
Hello CJ!
PA1961-1 has been cited in 117 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yan Zhang,Linchao Zhang,JiaLu Bao et al.Perfluorooctanoic Acid Exposure in Early Pregnancy Induces Oxidative Stress in The Uterus and Liver in Mice,13 February 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-160447
Species: Mouse
PA1961-1 usage in article: APP:IHC, SAMPLE:UTREUS, DILUTION:NA
Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
PA1961-1 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000
Jia-Hui Yan,Yi-Shun Ji,Man-Li Yang,Jun Fu,Hu Shan,Li−Li Wang,Lei Zhang,Jing-Yuan Xiao,Xiao-Ying Kong, and Jin-Sheng Shi ACS Applied Nano Materials 2020 3 (9), 8817-8828 DOI: 10.1021/acsanm.0c01607
Species: Cuttlefish,Human,Mouse
PA1961-1 usage in article: APP:IHC, SAMPLE:HEART,LIVER,SPLEEN,LUNG AND KIDNEY, DILUTION:NA
Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
PA1961-1 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:200
Gang Li,Jing Zhou,Mengyu Sun,Juren Cen,Jing Xu,Role of luteolin extracted from Clerodendrum cyrtophyllum Turcz leaves in protecting HepG2 cells from TBHP-induced oxidative stress and its cytotoxicity, genotoxicity,Journal of Functional Foods, Volume 74, 2
Species: Human
Fu Y,Wang Y,Gao X,Li H,Yuan Y.Dynamic Expression of HDAC3 in db/db Mouse RGCs and Its Relationship with Apoptosis and Autophagy.J Diabetes Res.2020 Mar 1;2020:6086780.doi:10.1155/2020/6086780.PMID: 32190700;PMCID:PMC7071812.
Species: Mouse
PA1961-1 usage in article: APP:IHC, SAMPLE:RENAL TISSUE, DILUTION:1:50
Yang X,Liu TC,Liu S,Zhu W,Li H,Liang P,Ye S,Cui S.Promoted Viability and Differentiated Phenotype of Cultured Chondrocytes With Low Level Laser Irradiation Potentiate Efficacious Cells for Therapeutics.Front Bioeng Biotechnol.2020 May 29;8:468.doi:10.3389
Species: White Rabbit
PA1961-1 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA
Sun K,Luo J,Jing X,Xiang W,Guo J,Yao X,Liang S,Guo F,Xu T.Hyperoside ameliorates the progression of osteoarthritis: An in vitro and in vivo study.Phytomedicine.2020 Oct 14;80:153387.doi:10.1016/j.phymed.2020. 153387.Epub ahead of print.PMID:33130473.
Species: Mouse
Wei JL,Wu CJ,Chen JJ,Shang FT,Guo SG,Zhang XC,Liu H.LncRNA NEAT1 promotes the progression of sepsis-induced myocardial cell injury by sponging miR-144-3p.Eur Rev Med Pharmacol Sci.2020 Jan;24(2):851-861.doi:10.26355/eurrev_202001_20069.PMID:32016991.
Species: Mouse
PA1961-1 usage in article: APP:WB, SAMPLE:HL-1 CELLS, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Caspase-3(P17)/CASP3 Antibody Picoband®
Question
I am interested in using your anti-Caspase-3(P17)/CASP3 antibody for response to lipopolysaccharide studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
Thank you for your inquiry. This PA1961-1 anti-Caspase-3(P17)/CASP3 antibody is validated on rat thymus tissue, heart tissue, tissue lysate, liver tissue, spleen tissue, mouse thymus tissue, lung tissue, brain tissue, testis tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-19
Question
We have seen staining in mouse lymph. Any tips? Is anti-Caspase-3(P17)/CASP3 antibody supposed to stain lymph positively?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
From what I have seen in literature lymph does express CASP3. From what I have seen in Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-25
Question
We purchased anti-Caspase-3(P17)/CASP3 antibody for IHC on t-cell last year. I am using rat, and We intend to use the antibody for WB next. I was wanting to use examining t-cell as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
I have checked the website and datasheets of our anti-Caspase-3(P17)/CASP3 antibody and it seems that PA1961-1 has been tested on rat in both IHC and WB. Thus PA1961-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-19
Question
We were content with the WB result of your anti-Caspase-3(P17)/CASP3 antibody. However we have been able to see positive staining in jejunal mucosa cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?
D. Zhao
Verified customer
Asked: 2017-02-22
Answer
From literature, jejunal mucosa does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-02-22
Question
We are currently using anti-Caspase-3(P17)/CASP3 antibody PA1961-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on dog tissues as well?
J. Zhao
Verified customer
Asked: 2014-10-31
Answer
The anti-Caspase-3(P17)/CASP3 antibody (PA1961-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-31