Click image to see more details
Product Info Summary
| SKU: | A01154-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cardiac Troponin T/TNNT2 Antibody Picoband®
SKU/Catalog Number
A01154-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cardiac Troponin T/TNNT2 Antibody Picoband® catalog # A01154-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cardiac Troponin T/TNNT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01154-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Cardiac Troponin T recombinant protein (Position: K88-K298).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01154-1 is reactive to TNNT2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
35.924kDa
Background of TNNT2
Cardiac muscle troponin T (cTnT), is a protein which in humans is encoded by the TNNT2 gene. It is mapped to 1q32.1. The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01154-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat heart tissue, rat heart tissue, mouse heart tissue, mouse heart tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody (A01154-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody (Catalog # A01154-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.
Protein Target Info & Infographic
Gene/Protein Information For TNNT2 (Source: Uniprot.org, NCBI)
Gene Name
TNNT2
Full Name
Troponin T, cardiac muscle
Weight
35.924kDa
Superfamily
troponin T family
Alternative Names
Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT; TNNT2 TNNT2 CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT troponin T2, cardiac type troponin T, cardiac muscle|cardiomyopathy, dilated 1D (autosomal dominant)|cardiomyopathy, hypertrophic 2|troponin T type 2 (cardiac)|truncated cardiac troponin T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNNT2, check out the TNNT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNNT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cardiac Troponin T/TNNT2 Antibody Picoband® (A01154-1)
Hello CJ!
No publications found for A01154-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cardiac Troponin T/TNNT2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cardiac Troponin T/TNNT2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Cardiac Troponin T/TNNT2 Antibody Picoband®
Question
We are currently using anti-Cardiac Troponin T/TNNT2 antibody A01154-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
The anti-Cardiac Troponin T/TNNT2 antibody (A01154-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-20
