Anti-NOD1 Antibody

Product Name

Anti-NOD1 Antibody

View all NOD1 Antibodies

SKU/Catalog Number

A00495

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NOD1 Antibody (Catalog # A00495). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NOD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NOD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00495)

Host

Rabbit

Contents

NOD1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NOD1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy end of human NOD1. The immunogen is located within the last 50 amino acids of NOD1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms are known to exist. The immunogen to this antibody is outside of the Integrin alpha4 recombinant protein fragment and thus will not recognize it.

Reactive Species

A00495 is reactive to NOD1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

107691 MW

Background of NOD1

NOD1 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD1 contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and ten tandem leucine-rich repeats (LRRs) in its C-terminus. The CARD is involved in apoptotic signaling, and NOD1 activates caspase-9 and NF-κB. LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00495 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NOD1 antibody can be used for detection of NOD1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For NOD1 (Source: Uniprot.org, NCBI)

Alternative Names

CARD4, NLRC1, CLR7.1, Caspase recruitment domain-containing protein 4, Nucleotide-binding oligomerization domain-containing protein 1 NOD1 CARD4, CLR7.1, NLRC1 nucleotide binding oligomerization domain containing 1 nucleotide-binding oligomerization domain-containing protein 1|NLR family, CARD domain containing 1|caspase recruitment domain family, member 4|caspase recruitment domain-containing protein 4|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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