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Product Info Summary
| SKU: | A01307 |
|---|---|
| Size: | 100µg |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Cannabinoid Receptor CB2 Cnr2 Antibody
View all Cannabinoid R2/CB2/CNR2 Antibodies
SKU/Catalog Number
A01307
Size
100µg
Form
Liquid
Description
Boster Bio Anti-Cannabinoid Receptor CB2 Cnr2 Antibody (Catalog# A01307). Tested in ELISA, IHC, WB application(s). This antibody reacts with Rat.
Storage & Handling
Store at -20°C for long-term storage. Keep on ice at all times.
Cite This Product
Anti-Cannabinoid Receptor CB2 Cnr2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01307)
Host
Rabbit
Contents
Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Synthetic peptide corresponding to the sequence near the N-terminus of rat cannabinoid receptor CB2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, horse, rhesus macaque, dog, chimpanzee.
Reactive Species
A01307 is reactive to Cnr2 in Rat
Reconstitution
Calculated molecular weight
72201 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01307 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~35kDa. Higher MW species (~50kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Membrane ELISA: Lewis rat spleen membranes (5µ
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Figure 1. Western blot analysis of Cnr2 using anti-Cnr2 antibody (A01307).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cnr2 antigen affinity purified polyclonal antibody (Catalog # A01307) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Cnr2.
Protein Target Info & Infographic
Gene/Protein Information For Cnr2 (Source: Uniprot.org, NCBI)
Gene Name
Cnr2
Full Name
Cannabinoid receptor 2
Weight
72201 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1;dNTPase;3.1.5.-;Dendritic cell-derived IFNG-induced protein;DCIP;Monocyte protein 5;MOP-5;SAM domain and HD domain-containing protein 1;SAMHD1;MOP5; CNR2 CB-2, CB2, CX5 cannabinoid receptor 2 cannabinoid receptor 2|cannabinoid receptor 2 (macrophage)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cnr2, check out the Cnr2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cnr2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cannabinoid Receptor CB2 Cnr2 Antibody (A01307)
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Customer Q&As
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3 Customer Q&As for Anti-Cannabinoid Receptor CB2 Cnr2 Antibody
Question
We are currently using anti-Cannabinoid Receptor CB2 antibody A01307 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
The anti-Cannabinoid Receptor CB2 antibody (A01307) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-06
Question
Would A01307 anti-Cannabinoid Receptor CB2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can see on the product datasheet, A01307 anti-Cannabinoid Receptor CB2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-01
Question
Is a blocking peptide available for product anti-Cannabinoid Receptor CB2 antibody (A01307)?
R. Wu
Verified customer
Asked: 2014-08-12
Answer
We do provide the blocking peptide for product anti-Cannabinoid Receptor CB2 antibody (A01307). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-08-12
