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Product Info Summary
| SKU: | PA1520 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CaMKK/CAMKK1 Antibody Picoband®
View all CaMKK alpha Antibodies
SKU/Catalog Number
PA1520
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CaMKK/CAMKK1 Antibody catalog # PA1520. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaMKK/CAMKK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1520)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CaMKK, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1520 is reactive to Camkk1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
55838 MW
Background of CaMKK alpha
CAMKK1, Calcium/calmodulin-dependent protein kinase kinase 1 is an enzyme that in humans is encoded by the CAMKK1 gene. The CAMKK1 gene is mapped to chromosome 17. The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.Camkk1binds calmodulin and activated Camk4 with a 6-fold increase in total activity and a 100-fold increase in Camk4 Ca (2+)-independent activity. Camkk1 catalyzed a 10-fold increase in the total activity of Camk1 and had no effect on Camk2. Cotransfection of COS-7 cells with Camkk1 and Camk4 resulted in a 14-fold increase in CRE-binding protein-dependent transcription compared with Camk4 alone, suggesting that Camkk1 enhances Camk4-mediated transcriptional regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1520 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Rat Brain Tissue, Mouse Brain Tissue, Mouse Brain Tissue,, rat brain tissue, mouse brain tissue, human U-87MG whole cell
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
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Anti-CaMKK antibody, PA1520, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
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Anti-CaMKK antibody, PA1520, IHC(P)
IHC(P): Rat Brain Tissue
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Figure 3. Western blot analysis of CAMKK1 using anti-CAMKK1 antibody (PA1520).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human U-87MG whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMKK1 antigen affinity purified polyclonal antibody (Catalog # PA1520) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAMKK1 at approximately 56KD. The expected band size for CAMKK1 is at 56KD.
Protein Target Info & Infographic
Gene/Protein Information For Camkk1 (Source: Uniprot.org, NCBI)
Gene Name
Camkk1
Full Name
Calcium/calmodulin-dependent protein kinase kinase 1
Weight
55838 MW
Superfamily
protein kinase superfamily
Alternative Names
Calcium/calmodulin-dependent protein kinase kinase 1;CaM-KK 1;CaM-kinase kinase 1;CaMKK 1;2.7.11.17;CaM-kinase IV kinase;Calcium/calmodulin-dependent protein kinase kinase alpha;CaM-KK alpha;CaM-kinase kinase alpha;CaMKK alpha;Camkk1;Camkk; Camkk1|AI846603, CaMKKalpha, Camkk, caM-KK 1, caM-KK alpha, caMKK 1, caMKK alpha|calcium/calmodulin-dependent protein kinase kinase 1, alpha|calcium/calmodulin-dependent protein kinase kinase 1|caM-kinase IV kinase|caM-kinase kinase 1|caM-kinase kinase alpha|calcium calmodulin dependent protein kinase kinase alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Camkk1, check out the Camkk1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Camkk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CaMKK/CAMKK1 Antibody Picoband® (PA1520)
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No publications found for PA1520
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Customer Q&As
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1 Customer Q&As for Anti-CaMKK/CAMKK1 Antibody Picoband®
Question
We are currently using anti-CaMKK/CAMKK1 antibody PA1520 for rat tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-CaMKK/CAMKK1 antibody (PA1520) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
