Product Info Summary
SKU: | A01905-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CAMKIV/Camk4 Antibody Picoband™
SKU/Catalog Number
A01905-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CAMKIV/Camk4 Antibody Picoband™ catalog # A01905-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAMKIV/Camk4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01905-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Camk4 recombinant protein (Position: A19-Y469).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01905-2 is reactive to CAMK4 in Mouse, Rat
Applications
A01905-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
58 kDa
Calculated molecular weight
40895 MW
Background of CaMKIV
Calcium/calmodulin-dependent protein kinase type IV is an enzyme that in humans is encoded by the CAMK4 gene. The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Immunofluorescence, 5μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
![a01905 2 camk4 primary antibodies wb testing 1 a01905 2 camk4 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of CAMKIV/Camk4 using anti-CAMKIV/Camk4 antibody (A01905-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMKIV/Camk4 antigen affinity purified polyclonal antibody (Catalog # A01905-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAMKIV/Camk4 at approximately 58 kDa. The expected band size for CAMKIV/Camk4 is at 58 kDa.
![a01905 2 camk4 primary antibodies ihc testing 2 a01905 2 camk4 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of CAMKIV/Camk4 using anti-CAMKIV/Camk4 antibody (A01905-2).
CAMKIV/Camk4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMKIV/Camk4 Antibody (A01905-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01905 2 camk4 primary antibodies ihc testing 3 a01905 2 camk4 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of CAMKIV/Camk4 using anti-CAMKIV/Camk4 antibody (A01905-2).
CAMKIV/Camk4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMKIV/Camk4 Antibody (A01905-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01905 2 camk4 primary antibodies ihc testing 4 a01905 2 camk4 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of CAMKIV/Camk4 using anti-CAMKIV/Camk4 antibody (A01905-2).
CAMKIV/Camk4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMKIV/Camk4 Antibody (A01905-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01905 2 camk4 primary antibodies ihc testing 5 a01905 2 camk4 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of CAMKIV/Camk4 using anti-CAMKIV/Camk4 antibody (A01905-2).
CAMKIV/Camk4 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMKIV/Camk4 Antibody (A01905-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a01905 2 camk4 primary antibodies if testing 6 a01905 2 camk4 primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of CAMKIV/Camk4 using anti-CAMKIV/Camk4 antibody (A01905-2).
CAMKIV/Camk4 was detected in an immunocytochemical section of RM-1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CAMKIV/Camk4 Antibody (A01905-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a01905 2 camk4 primary antibodies fcm testing 7 a01905 2 camk4 primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-fcm-testing-7.jpg)
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Figure 7. Flow Cytometry analysis of RAW264.7 cells using anti-CAMKIV/Camk4 antibody (A01905-2).
Overlay histogram showing RAW264.7 cells stained with A01905-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CAMKIV/Camk4 Antibody (A01905-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a01905 2 camk4 primary antibodies if testing 8 a01905 2 camk4 primary antibodies if testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a01905-2-camk4-primary-antibodies-if-testing-8.jpg)
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Figure 8. IF analysis of CAMKIV/Camk4 using anti-CAMKIV/Camk4 antibody (A01905-2).
CAMKIV/Camk4 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CAMKIV/Camk4 Antibody (A01905-2) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CAMK4 (Source: Uniprot.org, NCBI)
Gene Name
CAMK4
Full Name
Calcium/calmodulin-dependent protein kinase type IV
Weight
40895 MW
Superfamily
protein kinase superfamily
Alternative Names
brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; calcium/calmodulin-dependent protein kinase type IV; CAM kinase- GR; CAM kinase IV; CaM kinase-GR; CaMK IV; caMK; CaMK-GR; EC 2.7.11; EC 2.7.11.17; IV; MGC36771 CAMK4 CaMK IV, CaMK-GR, CaMKIV, caMK calcium/calmodulin dependent protein kinase IV calcium/calmodulin-dependent protein kinase type IV|CAM kinase IV|CAM kinase- GR|brain Ca(2+)-calmodulin-dependent protein kinase type IV|brain Ca++-calmodulin-dependent protein kinase type IV|calcium/calmodulin-dependent protein kinase type IV catalytic chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAMK4, check out the CAMK4 Infographic
![CAMK4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAMK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CAMKIV/Camk4 Antibody Picoband™ (A01905-2)
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