Click image to see more details
Product Info Summary
| SKU: | M02611 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CaMKII delta CAMK2D Rabbit Monoclonal Antibody
View all CaM Kinase II delta Antibodies
SKU/Catalog Number
M02611
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CaMKII delta CAMK2D Rabbit Monoclonal Antibody catalog # M02611. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaMKII delta CAMK2D Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02611)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABBF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CaMKII delta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02611 is reactive to CAMK2D in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
56369 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02611 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human Daudi whole cell, human U-87MG whole cell, human HUVEC whole cell, human A549 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CaMKII delta/CAMK2D using anti-CaMKII delta/CAMK2D antibody (M02611).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HUVEC whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII delta/CAMK2D antigen affinity purified monoclonal antibody (M02611) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII delta/CAMK2D at approximately 50 kDa. The expected band size for CaMKII delta/CAMK2D is at 56 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CAMK2D (Source: Uniprot.org, NCBI)
Gene Name
CAMK2D
Full Name
Calcium/calmodulin-dependent protein kinase type II subunit delta
Weight
56369 MW
Superfamily
protein kinase superfamily
Alternative Names
Calcium/calmodulin-dependent protein kinase type II subunit delta;CaM kinase II subunit delta;CaMK-II subunit delta;2.7.11.17;CAMK2D;CAMKD; CAMK2D CAMKD calcium/calmodulin dependent protein kinase II delta calcium/calmodulin-dependent protein kinase type II subunit delta|CaM kinase II delta subunit|CaM-kinase II delta chain|CaMK-II delta subunit|calcium/calmodulin-dependent protein kinase (CaM kinase) II delta|calcium/calmodulin-dependent protein kinase type II delta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAMK2D, check out the CAMK2D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAMK2D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CaMKII delta CAMK2D Rabbit Monoclonal Antibody (M02611)
Hello CJ!
No publications found for M02611
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CaMKII delta CAMK2D Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CaMKII delta CAMK2D Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-CaMKII delta CAMK2D Rabbit Monoclonal Antibody
Question
Does M02611 anti-CaMKII delta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
It shows on the product datasheet, M02611 anti-CaMKII delta Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-CaMKII delta Rabbit Monoclonal antibody M02611 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The anti-CaMKII delta Rabbit Monoclonal antibody (M02611) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-21
Question
Would anti-CaMKII delta Rabbit Monoclonal antibody M02611 work for WB with insulinoma?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
According to the expression profile of insulinoma, CAMK2D is highly expressed in insulinoma. So, it is likely that anti-CaMKII delta Rabbit Monoclonal antibody M02611 will work for WB with insulinoma.
Boster Scientific Support
Answered: 2019-10-09
Question
My question regarding product M02611, anti-CaMKII delta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02611 anti-CaMKII delta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-17
Question
Please see the WB image, lot number and protocol we used for insulinoma using anti-CaMKII delta Rabbit Monoclonal antibody M02611. Please let me know if you require anything else.
J. Brown
Verified customer
Asked: 2017-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-29
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for insulinoma using anti-CaMKII delta Rabbit Monoclonal antibody M02611. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-04
Question
Would anti-CaMKII delta Rabbit Monoclonal antibody M02611 work on canine WB with brain?
M. Moore
Verified customer
Asked: 2016-10-12
Answer
Our lab technicians have not tested anti-CaMKII delta Rabbit Monoclonal antibody M02611 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CaMKII delta Rabbit Monoclonal antibody M02611 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-12
