Anti-CAMK2 beta Antibody (C-term)

Product Name

Anti-CAMK2 beta Antibody (C-term)

View all CAMK2B Antibodies

SKU/Catalog Number

M03964-1

Size

50 µl

Description

Boster Bio Anti-CAMK2 beta Antibody (C-term) (Catalog # M03964-1). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-CAMK2 beta Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03964-1)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1050CT14.1.3

Isotype

IgG1

Immunogen

This CAMK2 beta antibody is generated from a mouse immunized with a recombinant protein between 1-503 amino acids from human CAMK2 beta.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03964-1 is reactive to CAMK2B in Human, Mouse, Rat

Applications

M03964-1 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

72678 Da

Background of CAMK2B

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-CAMK2 beta Antibody (C-term) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: PC-12 whole cell lysate
Lane 3: human brain lysate
Lane 4: mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 60 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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M03964-1 staining CAMK2 in human cerebellum tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For CAMK2B (Source: Uniprot.org, NCBI)

Alternative Names

calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase type II beta chain; calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II beta subunit; CaM kinase II subunit beta; CAM2CAMK2; CAMKB; CaMK-II subunit beta; CaM-kinase II beta chain; EC 2.7.11; EC 2.7.11.17; MGC29528; proline rich calmodulin-dependent protein kinase CAMK2B CAM2, CAMK2, CAMKB, CaMKIIbeta, MRD54 calcium/calmodulin dependent protein kinase II beta calcium/calmodulin-dependent protein kinase type II subunit beta|CaM kinase II beta subunit|CaM-kinase II beta chain|caMK-II subunit beta|proline rich calmodulin-dependent protein kinase

For more info on CAMK2B, check out the CAMK2B Infographic

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In this infographic, you will see the following information for CAMK2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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