Product Info Summary
SKU: | A01905 |
---|---|
Size: | 100uL |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CaM Kinase IV CAMK4 Antibody
SKU/Catalog Number
A01905
Size
100uL
Form
Lyophilized
Description
Boster Bio Anti-CaM Kinase IV CAMK4 Antibody (Catalog # A01905). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-CaM Kinase IV CAMK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01905)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 300-325 of Human CaM Kinase IV protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01905 is reactive to CAMK4 in Human, Mouse, Rat
Applications
A01905 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
51926 MW
Background of CaMKIV
CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:25,000
IHC: 1:500
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CaM Kinase IV (arrowhead) expression in rat brain extract (lane 1), Jurkat cell lysates (lane 2), specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively) using rabbit anti-CaM Kinase IV polyclonal antibody (Catalog # A01905) at 1:1000.
Click image to see more details
CaM Kinase IV was detected in paraffin-embedded sections of human brain cortex tissues using rabbit anti-CaM Kinase IV polyclonal antibody (Catalog # A01905) at 1:500. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Protein Target Info & Infographic
Gene/Protein Information For CAMK4 (Source: Uniprot.org, NCBI)
Gene Name
CAMK4
Full Name
Calcium/calmodulin-dependent protein kinase type IV
Weight
51926 MW
Superfamily
protein kinase superfamily
Alternative Names
brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; calcium/calmodulin-dependent protein kinase type IV; CAM kinase- GR; CAM kinase IV; CaM kinase-GR; CaMK IV; caMK; CaMK-GR; EC 2.7.11; EC 2.7.11.17; IV; MGC36771 CAMK4 CaMK IV, CaMK-GR, CaMKIV, caMK calcium/calmodulin dependent protein kinase IV calcium/calmodulin-dependent protein kinase type IV|CAM kinase IV|CAM kinase- GR|brain Ca(2+)-calmodulin-dependent protein kinase type IV|brain Ca++-calmodulin-dependent protein kinase type IV|calcium/calmodulin-dependent protein kinase type IV catalytic chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAMK4, check out the CAMK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAMK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CaM Kinase IV CAMK4 Antibody (A01905)
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Customer Q&As
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5 Customer Q&As for Anti-CaM Kinase IV CAMK4 Antibody
Question
Is a blocking peptide available for product anti-CaM Kinase IV antibody (A01905)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
We do provide the blocking peptide for product anti-CaM Kinase IV antibody (A01905). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-16
Question
Does anti-CaM Kinase IV antibody A01905 work on zebrafish IHC with leukemic t-cell?
E. Banerjee
Verified customer
Asked: 2019-04-09
Answer
Our lab technicians have not validated anti-CaM Kinase IV antibody A01905 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CaM Kinase IV antibody A01905 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish leukemic t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-09
Question
Do you have a BSA free version of anti-CaM Kinase IV antibody A01905 available?
C. Parker
Verified customer
Asked: 2016-02-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CaM Kinase IV antibody A01905 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-24
Question
We are currently using anti-CaM Kinase IV antibody A01905 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
L. Mitchell
Verified customer
Asked: 2016-02-12
Answer
The anti-CaM Kinase IV antibody (A01905) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-12
Question
Would A01905 anti-CaM Kinase IV antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Brown
Verified customer
Asked: 2014-10-01
Answer
It shows on the product datasheet, A01905 anti-CaM Kinase IV antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-10-01