Click image to see more details
-
-
-
-
-
+4
Product Info Summary
| SKU: | M00337 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Calpastatin (CAST) Mouse Monoclonal Antibody [Clone ID: OTI1E7]
View all Calpastatin Antibodies
SKU/Catalog Number
M00337
Size
100 µl
Description
Boster Bio CAST (Calpastatin) mouse monoclonal antibody, clone OTI1E7 (formerly 1E7). Catalog# M00337. Tested in FC, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calpastatin (CAST) Mouse Monoclonal Antibody [Clone ID: OTI1E7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00337)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1E7
Isotype
IgG1
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Calpastatin, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by eleven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00337 is reactive to CAST in Human
Reconstitution
Observed Molecular Weight
84.8 kDa
Calculated molecular weight
76.573kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00337 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
Click image to see more details
HEK293T cells transfected with either CAST (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CAST antibody (M00337)
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CAST mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Click image to see more details
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAST (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAST.
Click image to see more details
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and CAST-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CAST monoclonal antibody M00337
Protein Target Info & Infographic
Gene/Protein Information For CAST (Source: Uniprot.org, NCBI)
Gene Name
CAST
Full Name
Calpastatin
Weight
76.573kDa
Superfamily
protease inhibitor I27 (calpastatin) family
Alternative Names
BS-17; Calpain inhibitor; calpastatin; MGC9402; Sperm BS-17 component CAST BS-17, PLACK calpastatin calpastatin|calpain inhibitor|sperm BS-17 component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAST, check out the CAST Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAST: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calpastatin (CAST) Mouse Monoclonal Antibody [Clone ID: OTI1E7] (M00337)
Hello CJ!
No publications found for M00337
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Calpastatin (CAST) Mouse Monoclonal Antibody [Clone ID: OTI1E7]?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Calpastatin (CAST) Mouse Monoclonal Antibody [Clone ID: OTI1E7]
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
