Anti-Calpain 12 CAPN12 Antibody

Calpain 12 antibody

Boster Bio Anti-Calpain 12 CAPN12 Antibody catalog # A17322. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A17322
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Calpain 12 CAPN12 Antibody

View all Calpain 12 Antibodies

SKU/Catalog Number

A17322

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Calpain 12 CAPN12 Antibody catalog # A17322. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calpain 12 CAPN12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17322)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

1B9C1

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human Calpain 12. at AA rangle: 190-270

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A17322 is reactive to Capn12 in Human

Applications

A17322 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

81037 MW

Background of Calpain 12

BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Capn12 (Source: Uniprot.org, NCBI)

Gene Name

Capn12

Full Name

Calpain-12

Weight

81037 MW

Superfamily

peptidase C2 family

Alternative Names

calcium-activated neutral proteinase 12; calpain 12; calpain-12; CANP 12; MGC20576

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Capn12, check out the Capn12 Infographic

Capn12 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Capn12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Calpain 12 CAPN12 Antibody

Question

Here is the WB image, lot number and protocol we used for adult mammalian kidney using anti-Calpain 12 antibody A17322. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-28

Question

Would A17322 anti-Calpain 12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

As indicated on the product datasheet, A17322 anti-Calpain 12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-17

Question

We are currently using anti-Calpain 12 antibody A17322 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

S. Wu

Verified customer

Asked: 2017-03-14

Answer

The anti-Calpain 12 antibody (A17322) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-03-14

Question

Is there a BSA free version of anti-Calpain 12 antibody A17322 available?

M. Jones

Verified customer

Asked: 2013-08-12

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Calpain 12 antibody A17322 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-08-12

Order DetailsPrice
A17322

100uL

$399

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A17322
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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