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Product Info Summary
| SKU: | M03372-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Calnexin CANX Rabbit Monoclonal Antibody
SKU/Catalog Number
M03372-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Calnexin CANX Rabbit Monoclonal Antibody catalog # M03372-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calnexin CANX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03372-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HOE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Calnexin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03372-1 is reactive to CANX in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
97 kDa
Calculated molecular weight
67568 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03372-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:100
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CANX using anti-CANX antibody (M03372-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CANX antigen affinity purified monoclonal antibody (Catalog # M03372-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CANX at approximately 97 kDa. The expected band size for CANX is at 68 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CANX (Source: Uniprot.org, NCBI)
Gene Name
CANX
Full Name
Calnexin
Weight
67568 MW
Superfamily
calreticulin family
Alternative Names
Calnexin;IP90;Major histocompatibility complex class I antigen-binding protein p88;p90;CANX; CANX CNX, IP90, P90 calnexin calnexin|epididymis secretory sperm binding protein|major histocompatibility complex class I -binding protein p88
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CANX, check out the CANX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CANX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calnexin CANX Rabbit Monoclonal Antibody (M03372-1)
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1 Customer Q&As for Anti-Calnexin CANX Rabbit Monoclonal Antibody
Question
We are currently using anti-Calnexin Rabbit Monoclonal antibody M03372-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The anti-Calnexin Rabbit Monoclonal antibody (M03372-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-03
