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Product Info Summary
| SKU: | MA1009 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rabbit |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)
View all Caldesmon/CALD1 Antibodies
SKU/Catalog Number
MA1009
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) catalog # MA1009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit.
Storage & Handling
At -20˚C for one year and at 4˚C for one month from date of receipt. Avoid repeated freezing and thawing.
Cite This Product
Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1009)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
hHCD
Isotype
Mouse IgG1
Immunogen
Human uterus smooth muscle extract.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1009 is reactive to Cald1 in Human, Mouse, Rabbit
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100 ug/ml
Observed Molecular Weight
61 kDa
Calculated molecular weight
60584 MW
Background of Caldesmon/CALD1
Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al. (1992) demonstrated that the human CDM gene is composed of 14 exons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1009 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rabbit, By Heat
Western blot, 0.25-0.5μg/ml, Human, mouse, rabbit
Positive Control
IHC: Human Mammary Cancer Tissue, Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
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Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Caldesmon(smooth) antibody, MA1009, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For Cald1 (Source: Uniprot.org, NCBI)
Gene Name
Cald1
Full Name
Caldesmon
Weight
60584 MW
Superfamily
caldesmon family
Alternative Names
Non-muscle caldesmon;CDM;L-caldesmon;Cald1; CALD1 CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22 caldesmon 1 caldesmon|testis secretory sperm-binding protein Li 227n
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cald1, check out the Cald1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cald1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) (MA1009)
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No publications found for MA1009
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)
Question
Would MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
It shows on the product datasheet, MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
Is this MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) reactive to the isotypes of CALD1?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
The immunogen of MA1009 anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) is Human uterus smooth muscle extract. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-03
Question
Would anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 work on primate WB with skin?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Our lab technicians have not tested anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-31
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-16
Question
I see that the anti-Caldesmon (Smooth) antibody (Monoclonal, hHCD) MA1009 works with WB, what is the protocol used to produce the result images on the product page?
E. Parker
Verified customer
Asked: 2016-10-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-10-07
