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Product Info Summary
| SKU: | PB9937 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Calcitonin/CALCA Antibody
SKU/Catalog Number
PB9937
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calcitonin/CALCA Antibody catalog # PB9937. Tested in IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calcitonin/CALCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9937)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Calcitonin, different from the related human sequence by four amino acids, and identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9937 is reactive to Calca in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa, 45 kDa
Calculated molecular weight
14065 MW
Background of CALCA
Calcitonin, also known as CALCA, is a peptide hormone synthesized by the parafollicular cells of the thyroid. It is mapped to 11p15.2. Calcitonin belongs to the calcitonin-like protein family. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9937 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Positive Control
IHC: mouse brain tissue, rat lung tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Calcitonin using anti-Calcitonin antibody (PB9937).
Calcitonin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calcitonin Antibody (PB9937) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of Calcitonin using anti-Calcitonin antibody (PB9937).
Calcitonin was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calcitonin Antibody (PB9937) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Calca (Source: Uniprot.org, NCBI)
Gene Name
Calca
Full Name
Calcitonin
Weight
14065 MW
Superfamily
calcitonin family
Alternative Names
Calcitonin gene-related peptide 1;Alpha-type CGRP;Calcitonin gene-related peptide I;CGRP-I;Calca;Calc; CALCA CALC1, CGRP, CGRP-I, CGRP-alpha, CGRP1, CT, KC, PCT calcitonin related polypeptide alpha calcitonin|calcitonin gene-related peptide 1|alpha-type CGRP|calcitonin 1|calcitonin gene-related peptide I|calcitonin/calcitonin-related polypeptide, alpha|katacalcin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Calca, check out the Calca Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Calca: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calcitonin/CALCA Antibody (PB9937)
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No publications found for PB9937
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Customer Q&As
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16 Customer Q&As for Anti-Calcitonin/CALCA Antibody
Question
We have observed staining in mouse dorsal root ganglion. What should we do? Is anti-Calcitonin/CALCA antibody supposed to stain dorsal root ganglion positively?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Based on literature dorsal root ganglion does express CALCA. Based on Uniprot.org, CALCA is expressed in dorsal root ganglion, thyroid carcinoma, pheochromocytoma, among other tissues. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2020-04-02
Question
Will PB9937 anti-Calcitonin/CALCA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
As indicated on the product datasheet, PB9937 anti-Calcitonin/CALCA antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for dorsal root ganglion using anti-Calcitonin/CALCA antibody PB9937. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
We were satisfied with the WB result of your anti-Calcitonin/CALCA antibody. However we have observed positive staining in pheochromocytoma secreted. using this antibody. Is that expected? Could you tell me where is CALCA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Based on literature, pheochromocytoma does express CALCA. Generally CALCA expresses in secreted. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2019-12-13
Question
My question regarding product PB9937, anti-Calcitonin/CALCA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9937 anti-Calcitonin/CALCA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-05
Question
Is a blocking peptide available for product anti-Calcitonin/CALCA antibody (PB9937)?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
We do provide the blocking peptide for product anti-Calcitonin/CALCA antibody (PB9937). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-25
Question
Is PB9937 suitable for immunostaining of mouse long bone nerve fibers?
Verified customer
Asked: 2019-10-06
Answer
Yes, Anti-Calcitonin/CALCA Antibody Picoband™ (PB9937) is suitable for the immunostaining of mouse long bone nerve fibers.
Boster Scientific Support
Answered: 2019-10-09
Question
Do you have a BSA free version of anti-Calcitonin/CALCA antibody PB9937 available?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calcitonin/CALCA antibody PB9937 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-27
Question
I have attached the WB image, lot number and protocol we used for dorsal root ganglion using anti-Calcitonin/CALCA antibody PB9937. Please let me know if you require anything else.
A. Jackson
Verified customer
Asked: 2019-09-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-06
Question
Is this PB9937 anti-Calcitonin/CALCA antibody reactive to the isotypes of CALCA?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The immunogen of PB9937 anti-Calcitonin/CALCA antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Calcitonin (83-119aa SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSEAF), different from the related human sequence by four amino acids, and identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-06
Question
I was wanting to use your anti-Calcitonin/CALCA antibody for IHC for rat dorsal root ganglion on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat dorsal root ganglion identification?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
It shows on the product datasheet, PB9937 anti-Calcitonin/CALCA antibody has been validated for IHC on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat dorsal root ganglion in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-07
Question
Would anti-Calcitonin/CALCA antibody PB9937 work for IHC with dorsal root ganglion?
D. Krishna
Verified customer
Asked: 2016-04-04
Answer
According to the expression profile of dorsal root ganglion, CALCA is highly expressed in dorsal root ganglion. So, it is likely that anti-Calcitonin/CALCA antibody PB9937 will work for IHC with dorsal root ganglion.
Boster Scientific Support
Answered: 2016-04-04
Question
My lab would like to test anti-Calcitonin/CALCA antibody PB9937 on rat dorsal root ganglion for research purposes, then I may be interested in using anti-Calcitonin/CALCA antibody PB9937 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Kulkarni
Verified customer
Asked: 2016-02-22
Answer
The products we sell, including anti-Calcitonin/CALCA antibody PB9937, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-22
Question
I see that the anti-Calcitonin/CALCA antibody PB9937 works with IHC, what is the protocol used to produce the result images on the product page?
J. Jones
Verified customer
Asked: 2015-04-08
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-04-08
Question
My lab would like using your anti-Calcitonin/CALCA antibody for feeding behavior studies. Has this antibody been tested with western blotting on mouse lung? We would like to see some validation images before ordering.
G. Moore
Verified customer
Asked: 2013-04-04
Answer
I appreciate your inquiry. This PB9937 anti-Calcitonin/CALCA antibody is validated on mouse lung. It is guaranteed to work for IHC in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-04-04
Question
We are currently using anti-Calcitonin/CALCA antibody PB9937 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on dog tissues as well?
J. Krishna
Verified customer
Asked: 2013-02-18
Answer
The anti-Calcitonin/CALCA antibody (PB9937) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-18
